|Positive WB detected in||HepG2 cells, HeLa cells, Jurkat cells, U-937 cells, RAW 264.7 cells|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
66590-1-Ig targets FOS in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat, mouse samples.
|Tested Reactivity||Human, rat, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||FOS fusion protein Ag24340|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||55-60 kDa|
|GenBank accession number||BC004490|
|Gene ID (NCBI)||2353|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
c-FOS, also named as Proto-oncogene c-Fos and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. FOS is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, FOS undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the FOS protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the FOS protein becomes nuclear. The calculated molecular weight of FOS is 40 kDa, but Phosphorylated FOS protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. Upregulation of c-fos mRNA in a neuron indicates recent activity
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