c-Fos Monoclonal antibody

• Featured Product
• KD/KO Validated

c-Fos Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

47

CloneNo.

1G2C5

Reviews (1)

Cat no : 66590-1-Ig

Synonyms

AP 1, C FOS, Cellular oncogene fos, c-Fos, FOS, G0S7, Proto oncogene c Fos

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Validation Data Gallery

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  WB analysis of HepG2 using 66590-1-Ig

  WB result of c-Fos antibody (66590-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Fos transfected HepG2 cells.

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  WB analysis using 66590-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66590-1-Ig (c-Fos antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66590-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66590-1-Ig (c-Fos antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66590-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66590-1-Ig (FOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 66590-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human c-Fos (66590-1-Ig, Clone:1G2C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, U-937 cells, K-562 cells, THP-1 cells, Jurkat cells, RAW 264.7 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 40 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Product Information

66590-1-Ig targets c-Fos in WB, IHC, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: c-Fos fusion protein Ag24340
• Full Name: FOS
• Calculated molecular weight: 41 kDa
• Observed molecular weight: 55-60 kDa
• GenBank accession number: BC004490
• Gene symbol: FOS
• Gene ID (NCBI): 2353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Fos, also named as FOS and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. c-Fos is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, c-Fos undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the c-Fos protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the c-FOS protein becomes nuclear. c-Fos is a very short-lived intracellular protein, which is very easy to degrade. The calculated molecular weight of c-Fos is 40 kDa, but Phosphorylated c-Fos protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-Fos is an indirect marker of neuronal activity because c-Fos is often expressed when neurons fire action potentials. Upregulation of c-Fos mRNA in a neuron indicates recent activity.

Publications

Species	Application	Title
mouse	IF	Curr Biol; Neural processing of the reward value of pleasant odorants.; Authors - Maëllie Midroit
human	WB	Theranostics; KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.; Authors - Chengwan Zhang ; KD Validated
human	WB	Cancer Lett; MicroRNA-490-3P targets CDK1 and inhibits ovarian epithelial carcinoma tumorigenesis and progression.; Authors - Shuo Chen
mouse	WB	J Nanobiotechnology; The ketone body β-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation.; Authors - Yanglin Wu
human	WB	Genomics; Integrative epigenomic profiling reveal AP-1 is a key regulator in intrahepatich cholangiocarcinoma.; Authors - Ke He
human	WB	Pharmacol Res; CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.; Authors - Guopin Pan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (01-21-2023)

Suitable for IHC in the paraffinised brain sections of mice (cortex). Samples were fixed in 4% and standard IHC procedure with antigen retrieval and DAB detection was performed. Antibody was incubated at 1:1000 dilution overnight at 4C. Provided a good specific signal in neurons that increased after restraint stress.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse CNS tissue

c-Fos Antibody Immunohistochemistry validation (1:1000 dilution) in Mouse CNS tissue (Cat no:66590-1-Ig)