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FOS Monoclonal antibody

FOS Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G2C5

Cat no : 66590-1-Ig

Synonyms

AP 1, C FOS, Cellular oncogene fos, C-FOS, FOS, G0S7, Proto oncogene c Fos



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, U-937 cells, RAW 264.7 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66590-1-Ig targets FOS in WB, IHC, IF,ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FOS fusion protein Ag24340
Full Name FOS
Calculated molecular weight 41 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC004490
Gene symbol FOS
Gene ID (NCBI) 2353
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

c-FOS, also named as Proto-oncogene c-Fos and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. FOS is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, FOS undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the FOS protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the FOS protein becomes nuclear. The calculated molecular weight of FOS is 40 kDa, but Phosphorylated FOS protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. Upregulation of c-fos mRNA in a neuron indicates recent activity

Protocols

Product Specific Protocols
WB protocol for FOS antibody 66590-1-IgDownload protocol
IHC protocol for FOS antibody 66590-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Aging (Albany NY)

H2S restores the cardioprotective effects of ischemic post-conditioning by upregulating HB-EGF/EGFR signaling.

Authors - Yuanzhou Zhang
humanWB

Cancer Med

LncRNA-SNHG15 enhances cell proliferation in colorectal cancer by inhibiting miR-338-3p.

Authors - Min Li
humanWB

Biomed Res Int

RBM23 Drives Hepatocellular Carcinoma by Activating NF-κB Signaling Pathway.

Authors - Hexu Han
mouseIHC

Med Sci Monit

Construction of a Mex3c Gene-Deficient Mouse Model to Study C-FOS Expression in Hypothalamic Nuclei and Observe Morphological Differences in Embryonic Neural Tube Development.

Authors - Yong Du
humanWB

Pregnancy Hypertens

LncRNA MALAT1 affects the migration and invasion of trophoblast cells by regulating FOS expression in early-onset preeclampsia.

Authors - Qin Li
ratWB,IF

Mol Pain

The preemptive analgesia of pre-electroacupuncture in rats with formalin-induced acute inflammatory pain.

Authors - Qing Liu