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c-Fos Monoclonal antibody
c-Fos Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG1
Human, rat, mouse and More (1)
WB, IHC, FC, ELISA
Cat no : 66590-1-Ig
|Positive WB detected in||HeLa cells, HepG2 cells, U-937 cells, K-562 cells, THP-1 cells, Jurkat cells, RAW 264.7 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 40 publications below|
|IHC||See 5 publications below|
|IF||See 5 publications below|
66590-1-Ig targets c-Fos in WB, IHC, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.
|Tested Reactivity||Human, rat, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||c-Fos fusion protein Ag24340|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||55-60 kDa|
|GenBank accession number||BC004490|
|Gene ID (NCBI)||2353|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
c-Fos, also named as FOS and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. c-Fos is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, c-Fos undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the c-Fos protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the c-FOS protein becomes nuclear. c-Fos is a very short-lived intracellular protein, which is very easy to degrade. The calculated molecular weight of c-Fos is 40 kDa, but Phosphorylated c-Fos protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-Fos is an indirect marker of neuronal activity because c-Fos is often expressed when neurons fire action potentials. Upregulation of c-Fos mRNA in a neuron indicates recent activity.
|Product Specific Protocols|
|WB protocol for c-Fos antibody 66590-1-Ig||Download protocol|
|FC protocol for c-Fos antibody 66590-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tatyana (Verified Customer) (01-21-2023)
Suitable for IHC in the paraffinised brain sections of mice (cortex). Samples were fixed in 4% and standard IHC procedure with antigen retrieval and DAB detection was performed. Antibody was incubated at 1:1000 dilution overnight at 4C. Provided a good specific signal in neurons that increased after restraint stress.