Tested Applications
| Positive WB detected in | A375 cells, A549 cells, Calu-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
12663-1-AP targets FOSL1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3344 Product name: Recombinant human FOSL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-271 aa of BC016648 Sequence: MFRDFGEPGPSSGNGGGYGGPAQPPAAAQAAQQKFHLVPSINTMSGSQELQWMVQPHFLGPSSYPRPLTYPQYSPPQPRPGVIRALGPPPGVRRRPCEQISPEEEERRRVRRERNKLAAAKCRNRRKELTDFLQAETDKLEDEKSGLQREIEELQKQKERLELVLEAHRPICKIPEGAKEGDTGSTSGTSSPPAPCRPVPCISLSPGPVLEPEALHTPTLMTTPSLTPFTPSLVFTYPSTPEPCASAHRKSSSSSGDPSSDPLGSPTLLAL Predict reactive species |
| Full Name | FOS-like antigen 1 |
| Calculated Molecular Weight | 271 aa, 29 kDa |
| Observed Molecular Weight | 30-40 kDa |
| GenBank Accession Number | BC016648 |
| Gene Symbol | FOSL1 |
| Gene ID (NCBI) | 8061 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15407 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOSL1 (FOS Like 1, AP-1 Transcription Factor Subunit) is a protein-coding gene that produces the Fos-related antigen 1 (FRA1) protein, a key component of the AP-1 transcription factor complex. It is heavily involved in regulating cell proliferation, differentiation, tumorigenesis, and metastasis, particularly in cancers like lung, pancreatic, and breast cancer. FOSL1 forms heterodimers with JUN family proteins to form the AP-1 complex, which binds to DNA to regulate gene expression. It is considered an oncologist in many solid tumors, driving metastasis, EMT, and resistance to chemotherapy and radiotherapy. High expression of FOSL1 is associated with poor prognosis and tumor aggressiveness. It is often found in the stroma of reproductive tissues, cartilage, and in tumor microenvironments.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOSL1 antibody 12663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
