FOXC1 Monoclonal antibody

• Featured Product
• KD/KO Validated

FOXC1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1F4E11

Cat no : 66568-1-Ig

Synonyms

ARA, FKHL7, forkhead box C1, Forkhead box protein C1, Forkhead related protein FKHL7, FOXC1, FREAC 3, FREAC3, IGDA, IHG1, IRID1, RIEG3

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Validation Data Gallery

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  WB analysis of HEK-293 using 66568-1-Ig

  WB result of FOXC1 antibody (66568-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXC1 transfected HEK-293 cells.

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  WB analysis using 66568-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66568-1-Ig (FOXC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HEK293 cells, L02 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66568-1-Ig targets FOXC1 in WB,ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: forkhead box C1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: NM_001453
• Gene Symbol: FOXC1
• Gene ID (NCBI): 2296
• RRID: AB_2881929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXC1, also named as FKHL7 and FREAC3, binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3). Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). Defects in FOXC1 are a cause of Peters anomaly.