Ferritin heavy chain Recombinant monoclonal antibody

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• Ferritin heavy chain Recombinant monoclonal antibody (83428-1-RR)

• 

  WB analysis using 83428-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83428-1-RR (Ferritin heavy chain antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 83428-1-RR

  WB result of Ferritin heavy chain antibody (83428-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells.

• 

  WB analysis using 83428-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83428-1-RR (Ferritin heavy chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 83428-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (83428-1-RR, Clone: 230312F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 83428-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83428-1-RR against Human FTH1 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

Ferritin heavy chain Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83428-1-RR

Clone No.230312F3

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, ELISA

Synonyms

Ferritin, FTH1, 230312F3, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1

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Tested Applications

• Positive WB detected in: HCT 116 cells, HeLa cells, A549 cells, MDA-MB-231 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IF: See 2 publications below

Product Information

83428-1-RR targets Ferritin heavy chain in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2290

  Product name: Recombinant human FTH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-183 aa of BC000857

  Sequence: MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES

• Full Name: ferritin, heavy polypeptide 1
• Calculated Molecular Weight: 183 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC000857
• Gene Symbol: FTH1
• Gene ID (NCBI): 2495
• RRID: AB_3671075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P02794
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Viruses; Infectious Spleen and Kidney Necrosis Virus Triggers Ferroptosis in CPB Cells to Enhance Virus Replication; Authors - Qiushuang Zhang
human	WB	Cell Death Dis; SIRT5-mediated BCAT1 desuccinylation and stabilization leads to ferroptosis insensitivity and promotes cell proliferation in glioma; Authors - Tao Wang
mouse,human	WB,IF	Redox Biol; Dimethyl malonate preserves brain and neurobehavioral phenotype following neonatal hypoxia-ischemia by inhibiting FTH1-mediated ferritinophagy; Authors - Yiming Jin
rat,human	WB	FASEB J; TIMP3 Suppresses Oxidative Stress and Ferroptosis of Lens Epithelial Cells in Cataract Through Regulating the Keap1/Nrf2 Pathway.; Authors - Zhengyu Duan
mouse	WB	J Ethnopharmacol; Role of PTP1B in coordinating synaptic plasticity and ferroptosis: A mechanism underlying the antidepressant effect of spinosin from Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou.; Authors - Yanmei Wang
human	IF	Discov Oncol; Novel N(6)-methyladenosine (m(6)A) writer METTL16 promotes the cervical cancer tumorigenesis by targeting FTH1-dependent ferroptosis.; Authors - Zhisheng Huang