G0S2 Polyclonal antibody

• Featured Product
• KD/KO Validated

G0S2 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 12091-1-AP

Synonyms

G0/G1switch 2, G0S2, RP1 28O10.2

---

Validation Data Gallery

• 

  IHC staining of human liver using 12091-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12091-1-AP (G0S2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12091-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12091-1-AP (G0S2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of H9C2 using 12091-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using G0S2 antibody (12091-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Tested Applications

• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: H9C2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

12091-1-AP targets G0S2 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: G0S2 fusion protein Ag2731
• Full Name: G0/G1switch 2
• Calculated Molecular Weight: 11 kDa
• GenBank Accession Number: BC009694
• Gene Symbol: G0S2
• Gene ID (NCBI): 50486
• RRID: AB_2877824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G0S2 is G0/G1 switch protein 2. It is an all trans- retinoic acid (RA) target gene. G0S2 promotes apoptosis by binding to BCL2, hence preventing the formation of protective BCL2-BAX heterodimers.

Publications

Species	Application	Title
mouse	IHC	Cancer Res; Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.; Authors - Yogev Sela
human	WB	J Clin Endocrinol Metab; Endurance exercise training up-regulates lipolytic proteins and reduces triglyceride content in skeletal muscle of obese subjects.; Authors - Louche Katie K
human	IHC	Genomics; MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.; Authors - Yuan Shi
human	WB	J Clin Endocrinol Metab; Moderate-Intensity Exercise and High-Intensity Interval Training Affect Insulin Sensitivity Similarly in Obese Adults.; Authors - Benjamin J Ryan
human	WB	Mol Metab; G0/G1 Switch Gene 2 controls adipose triglyceride lipase activity and lipid metabolism in skeletal muscle.; Authors - Claire Laurens ; KD Validated
rat	WB	Cytokine; PMID: 32492632; Authors - Nguyen T T Truong