GAB2 Polyclonal antibody, PBS Only

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  WB analysis using 22549-1-AP (same clone as 22549-1-PBS)

  K-562 cells lysates were subjected to SDS PAGE followed by western blot with 22549-1-AP (GAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22549-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 22549-1-AP (same clone as 22549-1-PBS)

  WB result of GAB2 antibody (22549-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAB2 transfected K-562 cells. This data was developed using the same antibody clone with 22549-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 22549-1-AP (same clone as 22549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 22549-1-AP (GAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22549-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 22549-1-AP (same clone as 22549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 22549-1-AP (GAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22549-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 22549-1-AP (same clone as 22549-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GAB2 antibody (22549-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22549-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GAB2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 22549-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

22549-1-PBS targets GAB2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18192

  Product name: Recombinant human GAB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 111-450 aa of BC131711

  Sequence: SICQICGFNQAEESTDSLRNVSSAGHGPRSSPAELSSSSQHLLRERKSSAPSHSSQPTLFTFEPPVSNHMQPTLSTSAPQEYLYLHQCISRRAENARSASFSQGTRASFLMRSDTAVQKLAQGNGHCVNGISGQVHGFYSLPKPSRHNTEFRDSTYDLPRSLASHGHTKGSLTGSETDNEDVYTFKTPSNTLCREFGDLLVDNMDVPATPLSAYQIPRTFTLDKNHNAMTVATPGDSAIAPPPRPPKPSQAETPRWGSPQQRPPISENSRSVAATIPRRNTLPAMDNSRLHRASSCETYEYPQRGGESAGRSAESMSDGVGSFLPGKMIVGRSDSTNSED

• Full Name: GRB2-associated binding protein 2
• Calculated Molecular Weight: 676 aa, 74 kDa
• Observed Molecular Weight: 74-90 kDa
• GenBank Accession Number: BC131711
• Gene Symbol: GAB2
• Gene ID (NCBI): 9846
• RRID: AB_2879119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9UQC2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.