mouse brain tissue were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20089-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 20089-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
MCF7 cells were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 20089-1-AP
IP result of anti-GAB3-Specific (IP:20089-1-AP, 4ug; Detection:20089-1-AP 1:2000) with MCF-7 cells lysate 3200ug.
IP result of anti-GAB3-Specific (IP:20089-1-AP, 4ug; Detection:20089-1-AP 1:2000) with MCF-7 cells lysate 3200ug.
IHC staining of human liver cancer using 20089-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 20089-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of MCF-7 using 20089-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20089-1-AP (GAB3-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20089-1-AP (GAB3-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
20089-1-AP targets GAB3-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20089-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 20089-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 20089-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 20089-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 40x lens).
IP Figures
IP experiment of MCF-7 using 20089-1-AP
IP result of anti-GAB3-Specific (IP:20089-1-AP, 4ug; Detection:20089-1-AP 1:2000) with MCF-7 cells lysate 3200ug.
IF/ICC Figures
IF Staining of MCF-7 using 20089-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20089-1-AP (GAB3-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
GAB3-Specific Polyclonal antibody
Catalog Number
20089-1-AP
Citations
-
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
