GABRA4 Recombinant antibody

GABRA4 Recombinant Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

230302A4

Cat no : 83030-4-RR

Synonyms

GABRA4

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Validation Data Gallery

• 

  FC experiment of SH-SY5Y using 83030-4-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Anti-Human GABRA4 (83030-4-RR, Clone:230302A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of PC-3 using 83030-4-RR

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anti-Human GABRA4 (83030-4-RR, Clone:230302A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: PC-3 cells, SH-SY5Y cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83030-4-RR targets GABRA4 in FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GABRA4 fusion protein Ag3600
• Full Name: gamma-aminobutyric acid (GABA) A receptor, alpha 4
• Calculated Molecular Weight: 554 aa, 62 kDa
• Observed Molecular Weight: 62-70 kDa
• GenBank Accession Number: BC035055
• Gene Symbol: GABRA4
• Gene ID (NCBI): 2557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABRA4, encoding the extrasynaptically located α4‐subunit of the GABAA receptor, is involved in the etiology of autism. (PMID: 32028044, 32033586)