GALC Polyclonal antibody

GALC Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 51051-2-AP


Galactocerebrosidase, galactosylceramidase, GALC, GALCERase

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

51051-2-AP targets GALC in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALC fusion protein Ag0483
Full Name galactosylceramidase
Calculated Molecular Weight 668 aa, 76 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC036518
Gene Symbol GALC
Gene ID (NCBI) 2581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galactosylceramidase (Galc) is a lysosomal enzyme involved in the catabolism of galactosylceramide, a major lipid in myelin, kidney, and epithelial cells of the small intestine and colon. The normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits. Mutation of GALC gene will lead to Krabbe disease (globoid cell leukodystrophy), an autosomal recessive neurodegenerative disorder that affects both the central and peripheral nervous system due to an enzymatic defect of galactocerebrosidase. This antibody is specific to GALC.


Product Specific Protocols
WB protocol for GALC antibody 51051-2-APDownload protocol
IHC protocol for GALC antibody 51051-2-APDownload protocol
IF protocol for GALC antibody 51051-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Hum Genet

Chaperone therapy for Krabbe disease: potential for late-onset GALC mutations.

Authors - Mohammad Arif Hossain


Late-onset Krabbe disease is predominant in Japan and its mutant precursor protein undergoes more effective processing than the infantile-onset form.

Authors - Hossain Mohammad Arif MA

Biomater Adv

Micro/nano-patterns for enhancing differentiation of human neural stem cells and fabrication of nerve conduits via soft lithography and 3D printing

Authors - Jagoda Litowczenko