GALNS Polyclonal antibody

GALNS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14933-1-AP

Synonyms

Chondroitinase, Chondroitinsulfatase, Galactose 6 sulfate sulfatase, GALNAC6S, GalNAc6S sulfatase, GALNS, GAS, MPS4A

---

Validation Data Gallery

• 

  WB analysis using 14933-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14933-1-AP (GALNS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14933-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14933-1-AP (GALNS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14933-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14933-1-AP (GALNS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14933-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14933-1-AP (GALNS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, human liver tissue, HepG2 cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14933-1-AP targets GALNS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GALNS fusion protein Ag6746
• Full Name: galactosamine (N-acetyl)-6-sulfate sulfatase
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC050684
• Gene Symbol: GALNS
• Gene ID (NCBI): 2588
• RRID: AB_2108720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALNS(N-acetylgalactosamine-6-sulfatase) is also named as chondroitinase and belongs to the sulfatase family. It is one of sulfatases required to degrade glycosaminoglycans (GAGs), keratan sulfate (KS) and chondroitin-6-sulfate (C6S) and the enzyme is a dimer derived from two 60 kDa polypeptides, each of which is processed to 40 kDa and 15 kDa polypeptide subunits linked by disulfide bonds(PMID:15241807). The deduced 522-residue protein is composed of a 26-amino acid N-terminal signal peptide and a mature polypeptide of 496 amino acid residues, including 2 potential asparagine-linked glycosylation sites(PMID:1755850). Defects in GALNS are the cause of mucopolysaccharidosis type 4A (MPS4A), also known as Morquio A syndrome.

Publications

Species	Application	Title
human	IHC,IF	Discov Oncol; Identification of galactosamine-(N-acetyl)-6-sulfatase (GALNS) as a novel therapeutic target in progression of nasopharyngeal carcinoma; Authors - Jin Zhang