GALR2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87015-1-RR (same clone as 87015-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87015-1-RR (GALR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87015-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 87015-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87015-1-RR against Human GALR2 were performed. The affinity constant is 0.498 nM.

GALR2 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87015-1-PBS

Clone No.252067G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

GAL2 R, GAL2-R, galanin receptor 2, Galanin receptor type 2, GALNR2

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Product Information

87015-1-PBS targets GALR2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag24154

  Product name: Recombinant human GALR2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 287-387 aa of BC074915

  Sequence: VNPIVYALVSKHFRKGFRTICAGLLGRAPGRASGRVCAAARGTHSGSVLERESSDLLHMSEAAGALRPCPGASQPCILEPCPGPSWQGPKAGDSILTVDVA

• Full Name: galanin receptor 2
• Calculated Molecular Weight: 387 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC074915
• Gene Symbol: GALR2
• Gene ID (NCBI): 8811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43603
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Galanin receptor type 2 (GALR2) is a receptor for galanin, GALP, and the hormone SPX1 (PMID:24517231). It is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamo-pituitary axis. Activation of the GALR2 may protect the hippocampus from neuronal damage (PMID: 17263796). It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The activity of this receptor is mediated by G proteins that activate the phospholipase C/protein kinase C pathway (via G(q)) and that inhibits adenylyl cyclase. GALR2 has a calculated molecular weight of 42 kDa.