GATA3 Polyclonal antibody, PBS Only

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  WB analysis of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

  WB result of GATA3 antibody (22343-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA3 transfected MCF-7 cells. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 22343-1-AP (same clone as 22343-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22343-1-AP (same clone as 22343-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (22343-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GATA3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 22343-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GATA binding factor 3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3

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Product Information

22343-1-PBS targets GATA3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17833

  Product name: Recombinant human GATA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-262 aa of BC006793

  Sequence: MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGGASSSTHHPITTYPPYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTEGR

• Full Name: GATA binding protein 3
• Calculated Molecular Weight: 443 aa, 48 kDa
• Observed Molecular Weight: 37 kDa and 48 kDa
• GenBank Accession Number: BC006793
• Gene Symbol: GATA3
• Gene ID (NCBI): 2625
• RRID: AB_2879082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23771
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Trans-acting T-cell-specific transcription factor, also known as GATA-3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin and mammary gland (PMID: 25148023). GATA-3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full length GATA3 protein contains 444 aa corresponding to the molecular weights of 48,0 kDa, and the truncated protein was deteced at approximately 37 kDa (PMID: 24758297).