HeLa cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HUVEC using 67161-1-Ig
HUVEC cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
HUVEC cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 67161-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 67161-1-Ig
human placenta tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
human placenta tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of pig spleen using 67161-1-Ig
pig spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
pig spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 67161-1-Ig
rat spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
rat spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 67161-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 67161-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HeLa cells, human placenta tissue, HUVEC cells, pig spleen tissue, rat spleen tissue, HEK-293 cells
Positive IHC detected in
human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II IFNs and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.
HeLa cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HUVEC using 67161-1-Ig
HUVEC cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 67161-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 67161-1-Ig
human placenta tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of pig spleen using 67161-1-Ig
pig spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 67161-1-Ig
rat spleen tissue were subjected to SDS PAGE followed by western blot with 67161-1-Ig (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary tumor using 67161-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 67161-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67161-1-Ig (GBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
GBP1 Monoclonal antibody
Catalog Number
67161-1-Ig
Citations
3
Dilutions
WB : 1:5000-1:50000 IHC : 1:1000-1:4000
Applications
WB, IHC, ELISA
Reactivity
human, rat, pig, mouse, chinese tree shrews
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
