CoraLite® Plus 488-conjugated GBP1 Monoclonal antibody

GBP1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A10E8

Cat no : CL488-67161

Synonyms

GBP 1, GBP1, GTP binding protein 1, HuGBP 1



Tested Applications

Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-67161 targets GBP1 in IF applications and shows reactivity with Human, pig, rat samples.

Tested Reactivity Human, pig, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GBP1 fusion protein Ag7767
Full Name GTP binding protein 1
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC002666
Gene symbol GBP1
Gene ID (NCBI) 2633
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II IFNs and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GBP1 antibody CL488-67161Download protocol
Standard Protocols
Click here to view our Standard Protocols