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  • KD/KO Validated

GBP2 Polyclonal antibody

GBP2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, rat, mouse and More (1)





Cat no : 11854-1-AP


GBP 2, GBP2, GTP binding protein 2, HuGBP 2

Tested Applications

Positive WB detected inmouse spleen tissue, human placenta tissue, A375 cells, K-562 cells, human lung tissue, rat spleen tissue
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

11854-1-AP targets GBP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, Toxoplasma
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP2 fusion protein Ag2425
Full Name guanylate binding protein 2, interferon-inducible
Calculated molecular weight 591 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC022272
Gene symbol GBP2
Gene ID (NCBI) 2634
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness, because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)


Product Specific Protocols
WB protocol for GBP2 antibody 11854-1-APDownload protocol
IHC protocol for GBP2 antibody 11854-1-APDownload protocol
IF protocol for GBP2 antibody 11854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.

Authors - Peng Li


Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.

Authors - Etienne Meunier


IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.

Authors - Rajendra Karki


IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.

Authors - Si Ming Man

Nat Immunol

The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.

Authors - Si Ming Man

Nat Immunol

Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.

Authors - Etienne Meunier
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kyosuke (Verified Customer) (06-12-2019)

This antibody works very well for WB using HUVECs.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HUVECs