Validation Data Gallery
|Positive WB detected in
|mouse spleen tissue, human placenta tissue, A375 cells, K-562 cells, human lung tissue, rat spleen tissue
|Positive IHC detected in
|human spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11854-1-AP targets GBP2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
|human, rat, mouse
|human, mouse, rat, Toxoplasma
|Host / Isotype
|Rabbit / IgG
|GBP2 fusion protein Ag2425
|guanylate binding protein 2, interferon-inducible
|Calculated molecular weight
|591 aa, 67 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness, because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)
Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.
Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.
IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.
The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.
Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kyosuke (Verified Customer) (06-12-2019)
This antibody works very well for WB using HUVECs.