|Positive WB detected in||mouse spleen tissue, human placenta tissue, A375 cells, K-562 cells, human lung tissue, rat spleen tissue|
|Positive IHC detected in||human spleen tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11854-1-AP targets GBP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, Toxoplasma|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GBP2 fusion protein Ag2425|
|Full Name||guanylate binding protein 2, interferon-inducible|
|Calculated molecular weight||591 aa, 67 kDa|
|Observed molecular weight||67 kDa|
|GenBank accession number||BC022272|
|Gene ID (NCBI)||2634|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Brucella abortus Triggers a cGAS-Independent STING Pathway To Induce Host Protection That Involves Guanylate-Binding Proteins and Inflammasome Activation.
Life Sci Alliance
Initial phospholipid-dependent Irgb6 targeting to Toxoplasma gondii vacuoles mediates host defense.
GBP2 as a potential prognostic biomarker in pancreatic adenocarcinoma.
Life Sci Alliance
Cell-autonomous Toxoplasma killing program requires Irgm2 but not its microbe vacuolar localization.
Lipopolysaccharide restricts murine norovirus infection in macrophages mainly through NF-kB and JAK-STAT signaling pathway.
Galectin-3 regulates proinflammatory cytokine function and favours Brucella abortus chronic replication in macrophages and mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kyosuke (Verified Customer) (06-12-2019)
This antibody works very well for WB using HUVECs.