Tested Applications
| Positive WB detected in | mouse spleen tissue, human placenta tissue, A375 cells, K-562 cells, human lung tissue, rat spleen tissue |
| Positive IHC detected in | human spleen tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11854-1-AP targets GBP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, toxoplasma |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2425 Product name: Recombinant human GBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 132-481 aa of BC022272 Sequence: GTINQQAMDQLHYVTELTDRIKANSSPGNNSVDDSADFVSFFPAFVWTLRDFTLELEVDGEPITADDYLELSLKLRKGTDKKSKSFNDPRLCIRKFFPKRKCFVFDWPAPKKYLAHLEQLKEEELNPDFIEQVAEFCSYILSHSNVKTLSGGIPVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVEKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFMKNSFKDVDQMFQRKLGAQLEARRDDFCKQNSKASSDCCMALLQDIFGPLEEDVKQGTFSKPGGYRLFTQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLS Predict reactive species |
| Full Name | guanylate binding protein 2, interferon-inducible |
| Calculated Molecular Weight | 591 aa, 67 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC022272 |
| Gene Symbol | GBP2 |
| Gene ID (NCBI) | 2634 |
| RRID | AB_2109336 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P32456 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GBP2 antibody 11854-1-AP | Download protocol |
| IHC protocol for GBP2 antibody 11854-1-AP | Download protocol |
| WB protocol for GBP2 antibody 11854-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is the anti-GBP2 (Guanylate-Binding Protein 2) antibody, catalog number 11854-1-AP, with a total of 85 citations. Citations appear in high-impact journals including Nature, Cell, Cancer Cell, Nature Immunology, Nature Cell Biology, Nature Neuroscience, Nature Microbiology, Nature Communications, EMBO Journal, PNAS, Oncogene, Cell Reports, Journal of Immunology, and mBio, among others. Research fields span innate immunity, inflammasome activation, host defense against pathogens (Toxoplasma, Francisella, viruses), cancer biology, neuroinflammation, and ferroptosis.
| Species | Application | Title |
|---|---|---|
Nature Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. | ||
Nature Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence. | ||
Cancer Cell Intersection of immune and oncometabolic pathways drives cancer hyperprogression during immunotherapy | ||
Cell IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes. | ||
Nat Immunol The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection. |
Reviews
What customers say
The single review reports that this antibody performs very well for Western Blot using HUVECs, with a 1/1000 dilution. The reviewer gave it a 5-star rating, indicating strong satisfaction with its performance in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kyosuke (Verified Customer) (06-12-2019) | This antibody works very well for WB using HUVECs.
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