- Featured Product
- KD/KO Validated
GBP2 Polyclonal antibody
GBP2 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11854-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse spleen tissue, human placenta tissue, A375 cells, K-562 cells, human lung tissue, rat spleen tissue |
Positive IHC detected in | human spleen tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 22 publications below |
IHC | See 4 publications below |
IF | See 11 publications below |
Product Information
11854-1-AP targets GBP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse, Toxoplasma |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GBP2 fusion protein Ag2425 |
Full Name | guanylate binding protein 2, interferon-inducible |
Calculated molecular weight | 591 aa, 67 kDa |
Observed molecular weight | 67 kDa |
GenBank accession number | BC022272 |
Gene symbol | GBP2 |
Gene ID (NCBI) | 2634 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for GBP2 antibody 11854-1-AP | Download protocol |
IHC protocol for GBP2 antibody 11854-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Immunol Brucella abortus Triggers a cGAS-Independent STING Pathway To Induce Host Protection That Involves Guanylate-Binding Proteins and Inflammasome Activation. | ||
Life Sci Alliance Initial phospholipid-dependent Irgb6 targeting to Toxoplasma gondii vacuoles mediates host defense. | ||
Life Sci Alliance Cell-autonomous Toxoplasma killing program requires Irgm2 but not its microbe vacuolar localization. | ||
Virology Lipopolysaccharide restricts murine norovirus infection in macrophages mainly through NF-kB and JAK-STAT signaling pathway. | ||
Inflammation Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-γ. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kyosuke (Verified Customer) (06-12-2019) | This antibody works very well for WB using HUVECs.
|