Validation Data Gallery
|Positive WB detected in||mouse spleen tissue, human placenta tissue, A375 cells, K-562 cells, human lung tissue, rat spleen tissue|
|Positive IHC detected in||human spleen tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11854-1-AP targets GBP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, Toxoplasma|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GBP2 fusion protein Ag2425|
|Full Name||guanylate binding protein 2, interferon-inducible|
|Calculated molecular weight||591 aa, 67 kDa|
|Observed molecular weight||67 kDa|
|GenBank accession number||BC022272|
|Gene ID (NCBI)||2634|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Brucella abortus Triggers a cGAS-Independent STING Pathway To Induce Host Protection That Involves Guanylate-Binding Proteins and Inflammasome Activation.
Life Sci Alliance
Initial phospholipid-dependent Irgb6 targeting to Toxoplasma gondii vacuoles mediates host defense.
GBP2 as a potential prognostic biomarker in pancreatic adenocarcinoma.
Life Sci Alliance
Cell-autonomous Toxoplasma killing program requires Irgm2 but not its microbe vacuolar localization.
Lipopolysaccharide restricts murine norovirus infection in macrophages mainly through NF-kB and JAK-STAT signaling pathway.
Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-γ.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kyosuke (Verified Customer) (06-12-2019)
This antibody works very well for WB using HUVECs.