CoraLite® Plus 647-conjugated GBP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GBP2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-11854

Synonyms

GBP 2, GBP2, GTP binding protein 2, HuGBP 2

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Validation Data Gallery

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  FC experiment of HepG2 using CL647-11854

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human GBP2 (CL647-11854) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-11854 targets GBP2 in FC (Intra) applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBP2 fusion protein Ag2425
• Full Name: guanylate binding protein 2, interferon-inducible
• Calculated Molecular Weight: 591 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC022272
• Gene Symbol: GBP2
• Gene ID (NCBI): 2634
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness, because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C