GCK Monoclonal antibody, PBS Only

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  WB analysis of pig liver using 67216-1-Ig (same clone as 67216-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67216-1-Ig (same clone as 67216-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67216-1-Ig (same clone as 67216-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67216-1-Ig (same clone as 67216-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 67216-1-Ig (same clone as 67216-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67216-1-Ig (same clone as 67216-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 67216-1-Ig (same clone as 67216-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67216-1-Ig (same clone as 67216-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

GCK Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67216-1-PBS

Clone No.1C3A3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

HK IV, Hexokinase-D, Hexokinase-4, Glucokinase, EC:2.7.1.1

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Product Information

67216-1-PBS targets GCK in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8116

  Product name: Recombinant human GCK protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 157-465 aa of BC001890

  Sequence: EDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

• Full Name: glucokinase (hexokinase 4)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC001890
• Gene Symbol: GCK
• Gene ID (NCBI): 2645
• RRID: AB_2882508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P35557
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.