GCK Monoclonal antibody

GCK Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C3A3

Cat no : 67216-1-Ig

Synonyms

GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2



Tested Applications

Positive WB detected inpig liver tissue, SMMC-7721 cells, rat liver tissue, HSC-T6 cells, L02 cells, HepG2 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:300-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67216-1-Ig targets GCK in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GCK fusion protein Ag8116
Full Name glucokinase (hexokinase 4)
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC001890
Gene symbol GCK
Gene ID (NCBI) 2645
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.

Protocols

Product Specific Protocols
WB protocol for GCK antibody 67216-1-IgDownload protocol
IHC protocol for GCK antibody 67216-1-IgDownload protocol
IF protocol for GCK antibody 67216-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols