Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, L02 cells, mouse liver tissue, rat liver tissue, HepG2 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human liver cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 ul suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14241-1-AP targets GCLM in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5497 Product name: Recombinant human GCLM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-274 aa of BC041809 Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS Predict reactive species |
| Full Name | glutamate-cysteine ligase, modifier subunit |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC041809 |
| Gene Symbol | GCLM |
| Gene ID (NCBI) | 2730 |
| RRID | AB_2107832 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48507 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GCLM antibody 14241-1-AP | Download protocol |
| IF protocol for GCLM antibody 14241-1-AP | Download protocol |
| IHC protocol for GCLM antibody 14241-1-AP | Download protocol |
| IP protocol for GCLM antibody 14241-1-AP | Download protocol |
| WB protocol for GCLM antibody 14241-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GCLM Polyclonal antibody (Cat# 14241-1-AP) has accumulated 185 citations across journals including Cell, Nature Communications, PNAS, Molecular Cell, Science Advances, Redox Biology, Cell Death & Disease, Hepatology, Journal of Ethnopharmacology, and Free Radical Biology & Medicine. Key research fields include oxidative stress and antioxidant defense, Nrf2/Keap1 signaling, ferroptosis, glutathione metabolism, liver injury, neurodegeneration, cancer biology, inflammation, kidney and lung injury, pharmacology, and food science.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Modification of platinum sensitivity by KEAP1/NRF2 signals in non-small cell lung cancer. | ||
Nat Commun Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection. | ||
Nat Commun Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents. | ||
Hepatology Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury. | ||
J Adv Res Tussilagone attenuated cigarette smoke-induced chronic obstructive pulmonary disease through regulating Nrf2 and NF-κB/NLRP3 inflammasome via directly targeting cysteine 434 of KEAP1. |
Reviews
What customers say
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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Md Asad (Verified Customer) (02-24-2026) | This antibody gives very sharp bands in western blot.
|
Hua (Verified Customer) (01-17-2023) | Good antibody for WB. There are some non-specific bands.
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