GCLM Polyclonal antibody

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  WB analysis using 14241-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14241-1-AP (GCLM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14241-1-AP

  WB result of GCLM antibody (14241-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells.

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  WB analysis using 14241-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14241-1-AP (GCLM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 14241-1-AP

  IP result of anti-GCLM (IP:14241-1-AP, 4ug; Detection:14241-1-AP 1:2000) with A431 cells lysate 1000 ug.

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  IHC staining of human liver cancer using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14241-1-AP (GCLM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14241-1-AP (GCLM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 14241-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14241-1-AP (GCLM antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14241-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GCLM antibody (14241-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 14241-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GCLM Polyclonal antibody (14241-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

GCLM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14241-1-AP

Publications(152)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GLCLR, Glutamate--cysteine ligase modifier subunit, Glutamate--cysteine ligase regulatory subunit

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, L02 cells, mouse liver tissue, rat liver tissue, HepG2 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human liver cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 144 publications below
• IHC: See 10 publications below
• IF: See 6 publications below

Product Information

14241-1-AP targets GCLM in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5497

  Product name: Recombinant human GCLM protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-274 aa of BC041809

  Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

• Full Name: glutamate-cysteine ligase, modifier subunit
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC041809
• Gene Symbol: GCLM
• Gene ID (NCBI): 2730
• RRID: AB_2107832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48507
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun
mouse	WB	Acta Pharm Sin B; Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis; Authors - Yujun Zhou
human	WB	J Clin Invest; Identification of CD84 as a potent survival factor in acute myeloid leukemia; Authors - Yinghui Zhu
mouse	WB	Sci Adv; Immunomodulatory actions of a kynurenine-derived endogenous electrophile.; Authors - Mara Carreño

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (01-17-2023)

Good antibody for WB. There are some non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hela, human liver and mouse liver

GCLM Antibody Western Blot validation (1:2000 dilution) in Hela, human liver and mouse liver (Cat no:14241-1-AP)