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GCLM Polyclonal antibody
GCLM Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 14241-1-AP
Validation Data Gallery
|Positive WB detected in
|A431 cells, HeLa cells, L02 cells, mouse liver tissue, rat liver tissue, HepG2 cells
|Positive IP detected in
|Positive IHC detected in
|human liver cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:400-1:1600
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14241-1-AP targets GCLM in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, goat
|Host / Isotype
|Rabbit / IgG
|GCLM fusion protein Ag5497
|glutamate-cysteine ligase, modifier subunit
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.
|Product Specific Protocols
|WB protocol for GCLM antibody 14241-1-AP
|IHC protocol for GCLM antibody 14241-1-AP
|IF protocol for GCLM antibody 14241-1-AP
|IP protocol for GCLM antibody 14241-1-AP
|FC protocol for GCLM antibody 14241-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hua (Verified Customer) (01-17-2023)
Good antibody for WB. There are some non-specific bands.