Validation Data Gallery
|Positive WB detected in||A431 cells, MCF-7 cells, HeLa cells, L02 cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human liver cancer tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14241-1-AP targets GCLM in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GCLM fusion protein Ag5497|
|Full Name||glutamate-cysteine ligase, modifier subunit|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC041809|
|Gene ID (NCBI)||2730|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.
|Product Specific Protocols|
|WB protocol for GCLM antibody 14241-1-AP||Download protocol|
|IHC protocol for GCLM antibody 14241-1-AP||Download protocol|
|IF protocol for GCLM antibody 14241-1-AP||Download protocol|
|IP protocol for GCLM antibody 14241-1-AP||Download protocol|
|FC protocol for GCLM antibody 14241-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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