CoraLite® Plus 647-conjugated GCLM Polyclonal antibody
GCLM Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-14241
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-14241 targets GCLM in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GCLM fusion protein Ag5497 |
Full Name | glutamate-cysteine ligase, modifier subunit |
Calculated Molecular Weight | 31 kDa |
Observed Molecular Weight | 31 kDa |
GenBank Accession Number | BC041809 |
Gene Symbol | GCLM |
Gene ID (NCBI) | 2730 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 647 GCLM antibody CL647-14241 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |