GEMIN8 Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 67871-1-Ig (same clone as 67871-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67871-1-Ig (same clone as 67871-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67871-1-Ig (same clone as 67871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67871-1-Ig (GEMIN8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67871-1-Ig (same clone as 67871-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GEMIN8 antibody (67871-1-Ig, Clone: 1A6B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (Blue). This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

GEMIN8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67871-1-PBS

Clone No.1A6B4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Gemin-8, Gemin 8, Gem-associated protein 8, FAM51A1, 1A6B4

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Product Information

67871-1-PBS targets GEMIN8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6774

  Product name: Recombinant human GEMIN8 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-242 aa of BC003607

  Sequence: MAAVKASTSKATRPWYSHPVYARYWQHYHQAMAWMQSHHNAYRKAVESCFNLPWYLPSALLPQSSYDNEAAYPQSFYDHHVAWQDYPCSSSHFRRSGQHPRYSSRIQASTKEDQALSKEEEMETESDAEVECDLSNMEITEELRQYFAETERHREERRRQQQLDAERLDSYVNADHDLYCNTRRSVEAPTERPGERRQAEMKRLYGDSAAKIQAMEAAVQLSFDKHCDRKQPKYWPVIPLKF

• Full Name: gem (nuclear organelle) associated protein 8
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC003607
• Gene Symbol: GEMIN8
• Gene ID (NCBI): 54960
• RRID: AB_2918629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9NWZ8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.