ChromoTek GFP Monoclonal antibody (3H9)

Rat monoclonal antibody [3H9] to Green Fluorescent Proteins

Host/IsoType

Rat/IgG2a

Specificity

GFP, eGFP, wtGFP, YFP, CFP

Applications

WB

Conjugate

Unconjugated

CloneNo.

3H9

Publications

262

Cat no : 3h9

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

---

Validation Data Gallery

• 

  Dilution series of EGFP in HEK 293T cell lysate Primary antibody: [3H9] 1:1,000 Secondary antibody: goat anti-rat Alexa Fluor® 488 1:1,000

---

Product Information

Rat monoclonal antibody [3H9] to Green Fluorescent Proteins

• Applications: WB
• Recommended Dilution: Western blot: recommended starting dilution 1:1,000; Note: GFP antibody [3H9] does not work in immunofluorescence.
• Clonality: Monoclonal
• Host/IsoType: Rat/IgG2a
• Specificity/Target: The antibody recognizes green fluorescent proteins derived from Aequorea victoria GFP, such as eGFP, wtGFP, YFP, or CFP.
• Purity: Protein A or G purified
• RRID: AB_10773374
• Conjugate: Unconjugated
• CloneNo.: 3H9
• Storage Buffer: PBS, 50% glycerol, preservative: 0.09% sodium azide.
• Storage Condition: Shipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment.

Publications

Application	Title
WB	PLoS One; Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff).; Authors - Laura Lee
WB	Int J Mol Sci; Resolution of Two Steps in Botulinum Neurotoxin Serotype A1 Light Chain Localization to the Intracellular Plasma Membrane.; Authors - Alexander Gardner
	Bio Protoc; An Affinity-directed Protein Missile (AdPROM) System for Targeted Destruction of Endogenous Proteins.; Authors - Thomas J Macartney
	Nat Plants; A distinct giant coat protein complex II vesicle population in Arabidopsis thaliana.; Authors - Baiying Li
WB	PLoS Genet; Mapping the degradation pathway of a disease-linked aspartoacylase variant.; Authors - Sarah K Gersing
	Cell Rep; Spliceosomal component PRP-40 is a central regulator of microexon splicing.; Authors - Bikash Choudhary