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  • KD/KO Validated

GGCX Polyclonal antibody

GGCX Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16209-1-AP

Synonyms

gamma glutamyl carboxylase, GC, GGCX, VKCFD1



Tested Applications

Positive WB detected inMCF7 cells, HEK-293 cells, HepG2 cells, L02 cells, MCF-7 cells, mouse liver tissue, PC-3 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16209-1-AP targets GGCX in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GGCX fusion protein Ag9185
Full Name gamma-glutamyl carboxylase
Calculated molecular weight 758 aa, 88 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC013979
Gene symbol GGCX
Gene ID (NCBI) 2677
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GGCX(Gamma-glutamyl carboxylase) is also named as GC and belongs to the vitamin K-dependent gamma-carboxylase family. This 94 kDa (including all modifications, such as the five N-linked glycosylations), is a 5-pass transmembrane protein and a key regulator of blood coagulation(PMID:20518534). It mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide. Defects in GGCX are a cause of combined deficiency of vitamin K-dependent clotting factors type 1 (VKCFD1) and pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD)(PMID:9845520;17110937).

Protocols

Product Specific Protocols
WB protocol for GGCX antibody 16209-1-APDownload protocol
IHC protocol for GGCX antibody 16209-1-APDownload protocol
IP protocol for GGCX antibody 16209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Divergent effects of vitamins K1 and K2 on triple negative breast cancer cells.

Authors - Sarah Beaudin
humanWB

Methods Enzymol

Functional Study of the Vitamin K Cycle Enzymes in Live Cells.

Authors - J-K Tie
  • KO Validated
humanWB

JCI Insight

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.

Authors - Julie Lacombe

Blood

Characterization of vitamin K-dependent carboxylase mutations that cause bleeding and nonbleeding disorders.

Authors - Jian-Ke Tie
mouseWB

J Cell Biol

GGCX and VKORC1 inhibit osteocalcin endocrine functions.

Authors - Mathieu Ferron