GINS2 Polyclonal antibody

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  WB analysis of Jurkat using 16247-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16247-1-AP (PSF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16247-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16247-1-AP (PSF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16247-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16247-1-AP (PSF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 16247-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16247-1-AP (PSF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16247-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16247-1-AP (PSF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 16247-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSF2 antibody (16247-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of Jurkat using 16247-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GINS2 Polyclonal antibody (16247-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

GINS2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 16247-1-AP

Publications(13)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PSF2, CGI-122, DC5, GINS 2, HSPC037

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Jurkat cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

16247-1-AP targets GINS2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9180

  Product name: Recombinant human GINS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-185 aa of BC010164

  Sequence: MDAAEVEFLAEKELVTIIPNFSLDKIYLIGGDLGPFNPGLPVEVPLWLAINLKQRQKCRLLPPEWMDVEKLEKMRDHERKEETFTPMPSPYYMELTKLLLNHASDNIPKADEIRTLVKDMWDTRIAKLRVSADSFVRQQEAHAKLDNLTLMEINTSGTFLTQALNHMYKLRTNLQPLESTQSQDF

• Full Name: GINS complex subunit 2 (Psf2 homolog)
• Calculated Molecular Weight: 185 aa, 21 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC010164
• Gene Symbol: GINS2
• Gene ID (NCBI): 51659
• RRID: AB_2111895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y248
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	WB	J Cell Biol; LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.; Authors - Yilin Fan ; KD Validated
human	WB	Cell Rep; Impaired p32 Regulation Caused by the Lymphoma-Prone RECQ4 Mutation Drives Mitochondrial Dysfunction.; Authors - Jiin-Tarng Wang
mouse	WB	Oncogene; Premature activation of Cdk1 leads to mitotic events in S phase and embryonic lethality.; Authors - Radoslaw Szmyd
human	WB	Mol Cell Biol; A Role for USP7 in DNA Replication.; Authors - Jagannathan Madhav M
human	WB	J Biol Chem; TIMELESS suppresses the accumulation of aberrant CDC45-MCM2-7-GINS replicative helicase complexes on human chromatin.; Authors - Xiaohua Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SHENGYAN (Verified Customer) (01-30-2025)

The antibody works great in western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Has been tested in 293T, HaCaT, 143B, Psn1, H460 human cell lines

GINS2 Antibody Western Blot validation (1:2000 dilution) in Has been tested in 293T, HaCaT, 143B, Psn1, H460 human cell lines (Cat no:16247-1-AP)