Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16247-1-AP targets GINS2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9180 Product name: Recombinant human GINS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-185 aa of BC010164 Sequence: MDAAEVEFLAEKELVTIIPNFSLDKIYLIGGDLGPFNPGLPVEVPLWLAINLKQRQKCRLLPPEWMDVEKLEKMRDHERKEETFTPMPSPYYMELTKLLLNHASDNIPKADEIRTLVKDMWDTRIAKLRVSADSFVRQQEAHAKLDNLTLMEINTSGTFLTQALNHMYKLRTNLQPLESTQSQDF Predict reactive species |
| Full Name | GINS complex subunit 2 (Psf2 homolog) |
| Calculated Molecular Weight | 185 aa, 21 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC010164 |
| Gene Symbol | GINS2 |
| Gene ID (NCBI) | 51659 |
| RRID | AB_2111895 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y248 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GINS2 antibody 16247-1-AP | Download protocol |
| IF protocol for GINS2 antibody 16247-1-AP | Download protocol |
| IHC protocol for GINS2 antibody 16247-1-AP | Download protocol |
| WB protocol for GINS2 antibody 16247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GINS2 Polyclonal antibody (Cat# 16247-1-AP) has 16 citations. These appear in journals including Nature Communications, Cell Reports, Cell Death & Disease, Oncogene, Frontiers in Immunology, Molecular Cancer Therapeutics, JCI Insight, Journal of Cell Biology, Neurochemistry International, Journal of Biological Chemistry, Journal of Cancer, Molecular Cell Biology, and Journal of Gastroenterology and Hepatology. Research fields span DNA replication, cell cycle control, replisome biology, and cancer (glioma, hepatocellular, oral squamous cell, and esophageal cancers), with applications mainly in WB, IF, IHC, and CoIP.
| Species | Application | Title |
|---|---|---|
Nat Commun Nuclear compartmentalization at the G1/S transition plays a key role in DNA replication control. | ||
Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation | ||
Cell Death Dis GINS2 regulates temozolomide chemosensitivity via the EGR1/ECT2 axis in gliomas
| ||
Oncogene Premature activation of Cdk1 leads to mitotic events in S phase and embryonic lethality. | ||
Cell Rep Increased replication origin firing links replication stress to whole chromosomal instability in human cancer | ||
Cell Rep Impaired p32 Regulation Caused by the Lymphoma-Prone RECQ4 Mutation Drives Mitochondrial Dysfunction. |
Reviews
What customers say
The single review for this antibody is highly positive, rated 5 stars. The reviewer reports excellent performance in Western Blot at a 1:2000 dilution, with successful validation across multiple human cell lines including 293T, HaCaT, 143B, Psn1, and H460. Overall, the feedback indicates reliable and strong results for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
SHENGYAN (Verified Customer) (01-30-2025) | The antibody works great in western blot.
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