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PSF2 Polyclonal antibody

PSF2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16247-1-AP

Synonyms

DC5, GINS complex subunit 2, GINS2, HSPC037, Pfs2, PSF2



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16247-1-AP targets PSF2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSF2 fusion protein Ag9180
Full Name GINS complex subunit 2 (Psf2 homolog)
Calculated Molecular Weight 185 aa, 21 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC010164
Gene Symbol GINS2
Gene ID (NCBI) 51659
RRIDAB_2111895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.

Protocols

Product Specific Protocols
WB protocol for PSF2 antibody 16247-1-APDownload protocol
IHC protocol for PSF2 antibody 16247-1-APDownload protocol
IF protocol for PSF2 antibody 16247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
humanWB

J Cell Biol

LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.

Authors - Yilin Fan
  • KD Validated
humanWB

Cell Rep

Impaired p32 Regulation Caused by the Lymphoma-Prone RECQ4 Mutation Drives Mitochondrial Dysfunction.

Authors - Jiin-Tarng Wang
mouseWB

Oncogene

Premature activation of Cdk1 leads to mitotic events in S phase and embryonic lethality.

Authors - Radoslaw Szmyd
humanWB

Mol Cell Biol

A Role for USP7 in DNA Replication.

Authors - Jagannathan Madhav M
humanWB

J Biol Chem

TIMELESS suppresses the accumulation of aberrant CDC45-MCM2-7-GINS replicative helicase complexes on human chromatin.

Authors - Xiaohua Xu