GMCL1 Polyclonal antibody

GMCL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15575-1-AP

Synonyms

BTBD13, GCL, GCL1, Germ cell less protein like 1, GMCL1

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Validation Data Gallery

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  WB analysis using 15575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15575-1-AP (GMCL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid using 15575-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

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  IHC staining of human thyroid using 15575-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 15575-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 15575-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human thyroid tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15575-1-AP targets GMCL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GMCL1 fusion protein Ag7929
• Full Name: germ cell-less homolog 1 (Drosophila)
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC007420
• Gene Symbol: GMCL1
• Gene ID (NCBI): 64395
• RRID: AB_2878153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMCL1, also named as GCL, functions in spermatogenesis. It enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport. In WB test, the antibody recognizes 55 kDa band in our detection.