GNAT1 Polyclonal antibody, PBS Only

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  WB analysis using 55167-1-AP (same clone as 55167-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55167-1-AP (GNAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye tissue using 55167-1-AP (same clone as 55167-1-PBS)

  WB results of GNAT1 antibody (55167-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye using 55167-1-AP (same clone as 55167-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 55167-1-AP (GNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 55167-1-AP (same clone as 55167-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 55167-1-AP (GNAT1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 55167-1-AP (same clone as 55167-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 55167-1-AP (GNAT1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 55167-1-AP (same clone as 55167-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 55167-1-AP (GNAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 55167-1-AP (same clone as 55167-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GNAT1 (55167-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

GNAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 55167-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

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Product Information

55167-1-PBS targets GNAT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: NM_000172
• Gene Symbol: GNAT1
• Gene ID (NCBI): 2779
• RRID: AB_10858788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11488
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GNAT1, also named as GNATR, belongs to the G-alpha family and G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3). This antibody is specific to GNAT1.