GNAT1 Polyclonal antibody

GNAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 55167-1-AP

Synonyms

Transducin alpha-1 chain, Transducin alpha 1 chain, Guanine nucleotide-binding protein G(t) subunit alpha-1, GNATR, GBT1

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Validation Data Gallery

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  WB analysis using 55167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55167-1-AP (GNAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye tissue using 55167-1-AP

  WB results of GNAT1 antibody (55167-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.

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  WB analysis of mouse eye using 55167-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 55167-1-AP (GNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 55167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 55167-1-AP (GNAT1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 55167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 55167-1-AP (GNAT1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 55167-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 55167-1-AP (GNAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 55167-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GNAT1 (55167-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse retina tissue, mouse eye tissue, rat retina tissue
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:750-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

55167-1-AP targets GNAT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: NM_000172
• Gene Symbol: GNAT1
• Gene ID (NCBI): 2779
• RRID: AB_10858788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAT1, also named as GNATR, belongs to the G-alpha family and G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3). This antibody is specific to GNAT1.

Publications

Species	Application	Title
		Front Cell Dev Biol; Leveraging Zebrafish to Study Retinal Degenerations.; Authors - Juan M Angueyra
mouse	WB,IF	FEBS J; Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice; Authors - Xiao Li
mouse	WB	iScience; Identification of early-onset photoreceptor degeneration in transgenic mouse models of Alzheimer's disease.; Authors - Jie Zhang
zebrafish	WB	J Biol Chem; Pathogenic Mutations in Retinitis Pigmentosa 2 Predominantly Result in Loss of RP2 Protein Stability in Human and Zebrafish.; Authors - Fei Liu
mouse	WB	Sci Rep; Loss of PRCD alters number and packaging density of rhodopsin in rod photoreceptor disc membranes.; Authors - Emily R Sechrest
mouse	IHC	eNeuro; Frmpd1 facilitates trafficking of G-protein transducin and modulates synaptic function in rod photoreceptors of mammalian retina; Authors - Christie K Campla