GNAZ Polyclonal antibody

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  WB analysis using 12758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12758-1-AP (GNAZ antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GNAZ Polyclonal Antibody for WB, ELISA

Cat No. 12758-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Guanine nucleotide-binding protein G(z) subunit alpha, Gz alpha, Gz-alpha

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12758-1-AP targets GNAZ in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3255

  Product name: Recombinant human GNAZ protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-355 aa of BC026342

  Sequence: MGCRQSSEEKEAARRSRRIDRHLRSESQRQRREIKLLLLGTSNSGKSTIVKQMKIIHSGGFNLEACKEYKPLIIYNAIDSLTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRRLWADQGAQACFSRSSEYHLEDNAAYYLNDLERIAAADYIPTVEDILRSRDMTTGIVENKFTFKELTFKMVDVGGQRSERKKWIHCFEGVTAIIFCVELSGYDLKLYEDNQTSRMAESLRLFDSICNNNWFINTSLILFLNKKDLLAEKIRRIPLTICFPEYKGQNTYEEAAVYIQRQFEDLNRNKETKEIYSHFTCATDTSNIQFVFDAVTDVIIQNNLKYIGLC

• Full Name: guanine nucleotide binding protein (G protein), alpha z polypeptide
• Calculated Molecular Weight: 355 aa, 41 kDa
• Observed Molecular Weight: 38-41 kDa
• GenBank Accession Number: BC026342
• Gene Symbol: GNAZ
• Gene ID (NCBI): 2781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19086
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide-binding protein G(z) subunit alpha(GNAZ) is a member of a G protein subfamily that mediates signal transduction in pertussis toxin-insensitive systems. Heterotrimeric G proteins consist of alpha (α), beta (β), and gamma (γ) subunits. G proteins act as molecular switches inside cells, functioning to relay and transmit signals from cell surface receptors to intracellular effectors(PMID: 35052777). GNAS transduces signals from G protein-coupled receptors (GPCR) to adenyl cyclase (AC), and finally regulates the expression of cyclic adenosine monophosphate (cAMP)(PMID: 32700107).