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- KD/KO Validated
GP73/GOLPH2 Polyclonal antibody
GP73/GOLPH2 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 15126-1-AP
|Positive WB detected in||LNCaP cells, HeLa cells, MDA-MB-231 cells, HuH-7 cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, human liver cancer tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
15126-1-AP targets GP73/GOLPH2 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GP73/GOLPH2 fusion protein Ag7207|
|Full Name||golgi membrane protein 1|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||73 kDa|
|GenBank accession number||BC001740|
|Gene ID (NCBI)||51280|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.
|Product Specific Protocols|
|WB protocol for GP73/GOLPH2 antibody 15126-1-AP||Download protocol|
|IHC protocol for GP73/GOLPH2 antibody 15126-1-AP||Download protocol|
|IF protocol for GP73/GOLPH2 antibody 15126-1-AP||Download protocol|
|IP protocol for GP73/GOLPH2 antibody 15126-1-AP||Download protocol|
|FC protocol for GP73/GOLPH2 antibody 15126-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Curr Alzheimer Res
RCAN1 Increases Aβ Generation by Promoting N-Glycosylation Via Oligosaccharyl Transferase.
Signal Transduct Target Ther
GOLM1 restricts colitis and colon tumorigenesis by ensuring Notch signaling equilibrium in intestinal homeostasis.
Expression and prognostic significance of Golgiglycoprotein73 (GP73) with Epithelial-mesenchymal transition (EMT) related molecules in Hepatocellular Carcinoma (HCC).
Biochem Biophys Res Commun
Golgi protein 73 facilitates the interaction of hepatitis C virus NS5A with apolipoprotein E to promote viral particle secretion.
Eur Rev Med Pharmacol Sci
LncRNA MALAT1 knockdown inhibits cell migration and invasion by suppressing autophagy through miR-384/GOLM1 axis in glioma.
GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.