|Positive WB detected in||LNCaP cells, HeLa cells, MDA-MB-231 cells, HuH-7 cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, human liver cancer tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
15126-1-AP targets GP73/GOLPH2 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||GP73/GOLPH2 fusion protein Ag7207|
|Full Name||golgi membrane protein 1|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||73 kDa|
|GenBank accession number||BC001740|
|Gene ID (NCBI)||51280|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.
|Product Specific Protocols|
|WB protocol for GP73/GOLPH2 antibody 15126-1-AP||Download protocol|
|IHC protocol for GP73/GOLPH2 antibody 15126-1-AP||Download protocol|
|IF protocol for GP73/GOLPH2 antibody 15126-1-AP||Download protocol|
|IP protocol for GP73/GOLPH2 antibody 15126-1-AP||Download protocol|
|FC protocol for GP73/GOLPH2 antibody 15126-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Curr Alzheimer Res
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Expression and prognostic significance of Golgiglycoprotein73 (GP73) with Epithelial-mesenchymal transition (EMT) related molecules in Hepatocellular Carcinoma (HCC).
Biochem Biophys Res Commun
Golgi protein 73 facilitates the interaction of hepatitis C virus NS5A with apolipoprotein E to promote viral particle secretion.
Eur Rev Med Pharmacol Sci
LncRNA MALAT1 knockdown inhibits cell migration and invasion by suppressing autophagy through miR-384/GOLM1 axis in glioma.
GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.
GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.