Tested Applications
| Positive WB detected in | LNCaP cells, HuH-7 cells, MDA-MB-231 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer tissue, human liver cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 13 publications below |
| IHC | See 4 publications below |
| IF | See 7 publications below |
Product Information
15126-1-AP targets GP73/GOLPH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7207 Product name: Recombinant human GOLM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 36-400 aa of BC001740 Sequence: SRSVDLQTRIMELEGRVRRAAAERGAVELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL Predict reactive species |
| Full Name | golgi membrane protein 1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC001740 |
| Gene Symbol | GP73 |
| Gene ID (NCBI) | 51280 |
| RRID | AB_2113330 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NBJ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| IHC protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| IP protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| WB protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Pathog GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.
| ||
J Cell Sci Rapid affinity purification of intracellular organelles using a twin strep tag. | ||
Chemistry Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction | ||
Int J Med Sci Causal Association of Golgi Protein 73 With Coronary Artery Disease: Evidence from Proteomics and Mendelian Randomization | ||
J Cancer GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.
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