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GP73/GOLPH2 Polyclonal antibody

GP73/GOLPH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15126-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

GOLM1, GOLPH2, GP73, GOLM 1, golgi protein 73kD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inLNCaP cells, HuH-7 cells, MDA-MB-231 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15126-1-AP targets GP73/GOLPH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GP73/GOLPH2 fusion protein Ag7207 Predict reactive species
Full Name golgi membrane protein 1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC001740
Gene Symbol GP73
Gene ID (NCBI) 51280
RRIDAB_2113330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBJ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IHC protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IF protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IP protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation

Authors - Wanxin Guo
humanWB

PLoS Pathog

GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.

Authors - Xuewu Zhang
  • KD Validated
humanWB

J Cell Sci

Rapid affinity purification of intracellular organelles using a twin strep tag.

Authors - Jian Xiong
humanIF

Chemistry

Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction

Authors - Sayan Kundu
mouseWB,IF

Int J Med Sci

Causal Association of Golgi Protein 73 With Coronary Artery Disease: Evidence from Proteomics and Mendelian Randomization

Authors - Yi-Fen Lin
humanWB,IHC,IF

J Cancer

GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.

Authors - Jia-Zhou Ye
  • KD Validated
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