GOLPH3L Polyclonal antibody

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  WB analysis using 23153-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23153-1-AP (GOLPH3L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 23153-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23153-1-AP (GOLPH3L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 23153-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23153-1-AP (GOLPH3L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GOLPH3L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23153-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Golgi phosphoprotein 3-like, GPP34R, GPP34-related protein

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Tested Applications

• Positive WB detected in: MCF-7 cells, SK-BR-3 cells, T-47D cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23153-1-AP targets GOLPH3L in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19537

  Product name: Recombinant human GOLPH3L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 190-285 aa of BC013870

  Sequence: VTNTTEKQRLVKKLQDSVLERWVNDPQRMDKRTLALLVLAHSSDVLENVFSSLTDDKYDVAMNRAKDLVELDPEVEGTKPSATEMIWAVLAAFNKS

• Full Name: golgi phosphoprotein 3-like
• Calculated Molecular Weight: 285 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC013870
• Gene Symbol: GOLPH3L
• Gene ID (NCBI): 55204
• RRID: AB_3669408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4A5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi phosphoprotein 3 (GOLPH3) is a highly conserved membrane protein that plays an important role in maintaining the structure of the Golgi apparatus and anterograde transport. GOLPH3L(golgi phosphoprotein 3-like) is a GOLPH3 paralogue found in all vertebrate genomes. Compared with the oncogene GOLPH3, little is known about the role and function of GOLPH3L. It has been reported that GOLPH3L is highly expressed in epithelial ovarian cancer tissues, and cervical cancer tissue and is up-regulated in human rhabdomyosarcoma (PMID: 26284141; 28261346; 21822541).