GOPC Polyclonal antibody

GOPC Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12163-1-AP

Synonyms

CAL, CFTR associated ligand, FIG, Fused in glioblastoma, GOPC, GOPC1, PIST



Tested Applications

Positive WB detected inHepG2 cells, PC-3 cells, mouse colon
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12163-1-AP targets GOPC in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOPC fusion protein Ag2804
Full Name golgi associated PDZ and coiled-coil motif containing
Calculated molecular weight 454 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC009553
Gene symbol GOPC
Gene ID (NCBI) 57120
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOPC (FIG/PIST/CAL) is a PDZ-domain scaffolding protein that regulates the trafficking of many integral membrane proteins, including cell surface receptors, ion channels and adhesion molecules. GOPC localizes to the trans-Golgi network (TGN) but not to the cis- or trans-Golgi cisternae, thus anti-GOPC can be used to label the TGN structure.

Protocols

Product Specific Protocols
WB protocol for GOPC antibody 12163-1-APDownload protocol
IHC protocol for GOPC antibody 12163-1-APDownload protocol
IF protocol for GOPC antibody 12163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols