GPA33 Polyclonal antibody

• 

  WB analysis using 25941-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25941-1-AP (GPA33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GPA33 Polyclonal Antibody for WB, ELISA

Cat No. 25941-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

A33, Cell surface A33 antigen, Glycoprotein A33, GPA33

---

Tested Applications

• Positive WB detected in: COLO 320 cells, HT-29 cells, mouse colon tissue, mouse small intestine tissue, rat colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25941-1-AP targets GPA33 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPA33 fusion protein Ag12655
• Full Name: glycoprotein A33 (transmembrane)
• Calculated Molecular Weight: 319 aa, 36 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC074830
• Gene Symbol: GPA33
• Gene ID (NCBI): 10223
• RRID: AB_3669509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99795
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycoprotein A33 (GPA33) is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 9862345; 9012807). It contains an extracellular region of 213 amino acids, a transmembrane domain, and a highly polar intracellular tail containing four consecutive cysteine residues (PMID: 9012807). GPA33 is specifically expressed on the basolateral surfaces of intestinal epithelial cells of all lineages and is found in 95% of primary and metastatic colorectal cancers (PMID: 10960348; 10950144; 21541536). GPA33 may have a role in cell adhesion and has been implicated in immune dysregulation (PMID: 9012807; 33728639).