GPHN Recombinant monoclonal antibody, PBS Only (Detector)

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  Cytometric bead array sample test of MP03251-1

  The mean Gephyrin/GPHN concentration was determined to be 79.1 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 58.2 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 23.1 ng/mL in Caco-2 cell extract based on a 1.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP03251-1

  Cytometric bead array standard curve of MP03251-1, Gephyrin/GPHN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87903-4-PBS. Detection antibody: 87903-3-PBS. Standard: Ag3370. Range: 0.625-80 ng/mL.

GPHN Uni-rAb^® Recombinant Antibody for Cytometric bead array, Indirect ELISA

Cat No. 87903-3-PBS

Clone No.260132A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA

Synonyms

Gephyrin, Gephyrin/GPHN, Domain E, Domain G, GEPH

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Product Information

87903-3-PBS targets GPHN as part of a matched antibody pair:

MP03251-1: 87903-4-PBS capture and 87903-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3370

  Product name: Recombinant human GPHN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-315 aa of BC030016

  Sequence: MATEGMILTNHDHQIRVGVLTVSDSCFRNLAEDRSGINLKDLVQDPSLLGGTISAYKIVPDEIEEIKETLIDWCDEKELNLILTTGGTGFAPRDVTPEATKEVIEREAPGMALAMLMGSLNVTPLGMLSRPVCGIRGKTLIINLPGSKKGSQECFQFILPALPHAIDLLRDAIVKVKEVHDELEDLPSPPPPLSPPPTTSPHKQTEDKGVQCEEEEEEKKDSGVASTEDSSSSHITAAAIAAKKHPFYTSPAVVMAHGEQPIPGLINYSHHSTDERIPDSIISRGVQVLPRDTASLSTTPSESPRAQATSRLSTA

• Full Name: gephyrin
• Calculated Molecular Weight: 769 aa, 83 kDa
• GenBank Accession Number: BC030016
• Gene Symbol: GPHN
• Gene ID (NCBI): 10243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQX3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.