Rat GPNMB Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85632-5-RR (same clone as 85632-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85632-5-RR (Osteoactivin/GPNMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85632-5-PBS in a different storage buffer formulation.

• 

  IP experiment of rat spleen using 85632-5-RR (same clone as 85632-5-PBS)

  IP result of anti-GPNMB (IP:85632-5-RR, 4ug; Detection:85632-5-RR 1:500) with rat spleen tissue lysate 2400 ug. This data was developed using the same antibody clone with 85632-5-PBS in a different storage buffer formulation.

GPNMB Uni-rAb^® Recombinant Antibody for WB, IP, Indirect ELISA

Cat No. 85632-5-PBS

Clone No.242601D1

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IP, Indirect ELISA

Synonyms

Osteoactivin

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Product Information

85632-5-PBS targets GPNMB in WB, IP, Indirect ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3283

  Product name: Recombinant Rat Osteoactivin/GPNMB protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 23-498 aa of NM_133298.1

  Sequence: KRFRDVLGHEQYPDHMRENNQLRGWSSDENEWDEQLYPVWRRGEGRWKDSWEGGRVQAALTSDSPALVGSNITFVVNLVFPRCQKEDANGNIVYERNCRSDLELASDPYVYNWTTGADDEDWEDNTSQGQHLRFPDGKPFPRPHGRKKWNFVYVFHTLGQYFQKLGQCSARVSINTVNLTVGPQVMEVIVFRRHGRAYIPISKVKDVYVITDQIPIFVTMYQKNDRNSSDETFLRDLPIFFDVLIHDPSHFLNYSAISYKWNFGDNTGLFVSNNHTLNHTYVLNGTFNFNLTVQTAVPGPCPSPTPSPSSSTSPSPASSPSPTLSTPSPSLMPTGYKSMELSDISNENCRINRYGYFRATITIVDGILEVNIIQVADVPIPTLQPDNSLMDFIVTCKGATPTEACTIISDPTCQIAQNRVCSPVAVDELCLLSVRRAFNGSGTYCVNFTLGDDASLALTSALISIPGKDLGSPLRT

• Full Name: glycoprotein (transmembrane) nmb
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 120 kDa, 95 kDa
• GenBank Accession Number: NM_133298.1
• Gene Symbol: Gpnmb
• Gene ID (NCBI): 113955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9QZF6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.