GPR81 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPR81 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 20146-1-AP

Synonyms

G-protein coupled receptor 81, G-protein coupled receptor 104, GPR104, G protein coupled receptor 81, G protein coupled receptor 104

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Validation Data Gallery

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  WB analysis using 20146-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20146-1-AP (GPR81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 20146-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 20146-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20146-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 20146-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 20146-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 20146-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20146-1-AP (GPR81 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue
• Positive IHC detected in: mouse pancreas tissue, human colon tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20146-1-AP targets GPR81 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR81 fusion protein Ag13682
• Full Name: G protein-coupled receptor 81
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC066881
• Gene Symbol: GPR81
• Gene ID (NCBI): 27198
• RRID: AB_2878647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR81 (G protein-coupled receptor 81) is one of a large family of GPRs with low affinity for hydroxy-carboxylic acid structure ligands (PMID: 24657625). Lactate functions as a signaling molecule by serving as an agonist for the GPR81, involving both autocrine and paracrine mechanisms (PMID: 31836453). Moreover, A higher molecular mass band (∼60 kDa) is present in the brain and adipose tissue. This band is also strong in the transfected HeLa cells but weak in native HeLa cells and is therefore probably a form of GPR81, possibly a glycosylated form of the receptor (PMID: 23696276).

Publications

Species	Application	Title
mouse	WB	Life Sci; Aerobic exercise regulates GPR81 signal pathway and mediates complement- microglia axis homeostasis on synaptic protection in the early stage of Alzheimer's disease; Authors - Jialun Yang
mouse	WB	Front Oncol; Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis; Authors - Man Luo ; KD Validated
rat	WB	Sci Rep; PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.; Authors - Jing Li
human	IHC,IF	Placenta; Immunohistochemical localization of HCA1 receptor in placenta in presence of fetal growth restriction; Authors - Ayumi Kozai
		Adv Sci (Weinh); Breed-Driven Microbiome Heterogeneity Regulates Intestinal Stem Cell Proliferation via Lactobacillus-Lactate-GPR81 Signaling; Authors - Haiqin Wu