Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue, mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16897-1-AP targets GPT / ALT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10424 Product name: Recombinant human GPT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 147-496 aa of BC018207 Sequence: IPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAWALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVYAAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQLPPRAVERAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFHAKFTLEYS Predict reactive species |
| Full Name | glutamic-pyruvate transaminase (alanine aminotransferase) |
| Calculated Molecular Weight | 496 aa, 55 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC018207 |
| Gene Symbol | ALT1 |
| Gene ID (NCBI) | 2875 |
| RRID | AB_2230815 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P24298 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPT / ALT1 antibody 16897-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GPT/ALT1 polyclonal antibody (Cat# 16897-1-AP) has 19 citations. Journals include Cell Metabolism, Cancer Discovery, Cancer Research, Journal of Clinical Investigation, EMBO Molecular Medicine, Cell Reports, Oncotarget, and others. Research fields span cancer metabolism (renal, pancreatic, ovarian, liver, and breast cancers), amino acid/glutamine metabolism, mitochondrial dysfunction, pulmonary fibrosis, Alzheimer's disease, acute lung injury, liver injury biomarkers, and metabolic reprogramming in immune and endocrine contexts.
| Species | Application | Title |
|---|---|---|
Cell Metab Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations. | ||
Cancer Discov Selective Alanine Transporter Utilization Creates a Targetable Metabolic Niche in Pancreatic Cancer. | ||
Cancer Discov Copper Drives Remodeling of Metabolic State and Progression of Clear Cell Renal Cell Carcinoma | ||
Cancer Res Selective Alanine Transporter Utilization is a Therapeutic Vulnerability in ARID1A-Mutant Ovarian Cancer | ||
J Clin Invest Glutamine synthetase limits b-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1 | ||
EMBO Mol Med A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy |
