GPT/ ALT1 Polyclonal antibody

GPT/ ALT1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16897-1-AP

Synonyms

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ ALT1, GPT1

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Validation Data Gallery

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  WB analysis using 16897-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16897-1-AP (GPT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 16897-1-AP

  IP Result of anti-GPT (IP:16897-1-AP, 4ug; Detection:16897-1-AP 1:500) with L02 cells lysate 1800ug.

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IP detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below

Product Information

16897-1-AP targets GPT/ ALT1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPT/ ALT1 fusion protein Ag10424
• Full Name: glutamic-pyruvate transaminase (alanine aminotransferase)
• Calculated molecular weight: 496 aa, 55 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC018207
• Gene symbol: GPT
• Gene ID (NCBI): 2875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

Publications

Species	Application	Title
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
mouse	WB	Cell Rep; T Cell Activation Depends on Extracellular Alanine.; Authors - Noga Ron-Harel
human	WB	Oncotarget; Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.; Authors - Santosh K Bharti
human, mouse	WB	J Proteome Res; Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.; Authors - Shizhen Qin
mouse	WB	Mediators Inflamm; Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.; Authors - Shuaiwei Wang
human,mouse	WB	Cancer Med; Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.; Authors - Xiaoxiao Cai