GPT / ALT1 Polyclonal antibody

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  WB analysis using 16897-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16897-1-AP (GPT/ ALT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

GPT / ALT1 Polyclonal Antibody for WB, ELISA

Cat No. 16897-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ ALT1, GPT1

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below

Product Information

16897-1-AP targets GPT / ALT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPT / ALT1 fusion protein Ag10424
• Full Name: glutamic-pyruvate transaminase (alanine aminotransferase)
• Calculated Molecular Weight: 496 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC018207
• Gene Symbol: ALT1
• Gene ID (NCBI): 2875
• RRID: AB_2230815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24298
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

Publications

Species	Application	Title
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
mouse	WB	Cell Rep; T Cell Activation Depends on Extracellular Alanine.; Authors - Noga Ron-Harel
human	WB	Oncotarget; Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.; Authors - Santosh K Bharti
human, mouse	WB	J Proteome Res; Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.; Authors - Shizhen Qin
mouse	WB	Mediators Inflamm; Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.; Authors - Shuaiwei Wang
human,mouse	WB	Cancer Med; Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.; Authors - Xiaoxiao Cai