|Positive WB detected in
|mouse liver tissue, rat liver tissue, mouse spleen tissue
|Positive IP detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16897-1-AP targets GPT / ALT1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|GPT / ALT1 fusion protein Ag10424
|glutamic-pyruvate transaminase (alanine aminotransferase)
|Calculated molecular weight
|496 aa, 55 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
T Cell Activation Depends on Extracellular Alanine.
Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.
J Proteome Res
Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.
Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.
Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.