GPT / ALT1 Polyclonal antibody

GPT / ALT1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16897-1-AP

Synonyms

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ ALT1, GPT1



Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue, mouse spleen tissue
Positive IP detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 10 publications below

Product Information

16897-1-AP targets GPT / ALT1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPT / ALT1 fusion protein Ag10424
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC018207
Gene symbol GPT
Gene ID (NCBI) 2875
RRIDAB_2230815
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

Protocols

Product Specific Protocols
WB protocol for GPT / ALT1 antibody 16897-1-APDownload protocol
IP protocol for GPT / ALT1 antibody 16897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Metab

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Authors - Qiuying Chen
mouseWB

Cell Rep

T Cell Activation Depends on Extracellular Alanine.

Authors - Noga Ron-Harel
humanWB

Oncotarget

Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.

Authors - Santosh K Bharti
human, mouseWB

J Proteome Res

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Authors - Shizhen Qin
mouseWB

Mediators Inflamm

Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.

Authors - Shuaiwei Wang
human,mouseWB

Cancer Med

Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.

Authors - Xiaoxiao Cai