GPT/ALT1 Monoclonal antibody

GPT/ALT1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1


Human, Pig, rat, mouse







Cat no : 67531-1-Ig


AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1

Tested Applications

Positive WB detected inhuman milk, pig heart tissue, pig liver tissue, rat liver tissue, mouse liver tissue, rabbit heart tissue
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67531-1-Ig targets GPT/ALT1 in WB, IF, ELISA applications and shows reactivity with Human, Pig, rat, mouse samples.

Tested Reactivity Human, Pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPT/ALT1 fusion protein Ag10453
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC018207
Gene Symbol GPT
Gene ID (NCBI) 2875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.


Product Specific Protocols
WB protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
IF protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols