GPT/ALT1 Monoclonal antibody

GPT/ALT1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

5F6B5

Cat no : 67531-1-Ig

Synonyms

AAT1, Alanine aminotransferase 1, ALT1, GPT, GPT 1, GPT1



Tested Applications

Positive WB detected inpig heart tissue, pig liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67531-1-Ig targets GPT/ALT1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPT/ALT1 fusion protein Ag10453
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC018207
Gene symbol GPT
Gene ID (NCBI) 2875
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

Protocols

Product Specific Protocols
WB protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
IHC protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
IF protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols