GRK5 Polyclonal antibody, PBS Only

• 

  WB analysis of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17032-1-AP (same clone as 17032-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17032-1-AP (same clone as 17032-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17032-1-AP (GRK5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17032-1-AP (GRK5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 17032-1-AP (same clone as 17032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17032-1-AP (GRK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 17032-1-AP (same clone as 17032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17032-1-AP (GRK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GRK5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17032-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

17032-1-PBS targets GRK5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10511

  Product name: Recombinant human GRK5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 186-590 aa of BC064506

  Sequence: FRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSSKTSFNHHINSNHVSSNSTGSS

• Full Name: G protein-coupled receptor kinase 5
• Calculated Molecular Weight: 590 aa, 68 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC064506
• Gene Symbol: GRK5
• Gene ID (NCBI): 2869
• RRID: AB_2115453
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P34947
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GRK5, also named as GPRK5, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. It is Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. GRK5 is crucial for proper neuronal morphogenesis and the establishment of functional neuronal circuitry.