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GRP78/BIP Polyclonal antibody
GRP78/BIP Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IP, IHC, IF, FC, CoIP,ELISA
Conjugate
Unconjugated
246
Cat no : 11587-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | mouse kidney tissue, human breast cancer tissue, human pancreas cancer tissue, mouse heart tissue, rat brain tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 213 publications below |
| IHC | See 31 publications below |
| IF | See 26 publications below |
| IP | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
11587-1-AP targets GRP78/BIP in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | bovine, chicken, deer, fish, Hamster, human, mouse, pig, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRP78/BIP fusion protein Ag2188 |
| Full Name | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 70-78 kDa |
| GenBank accession number | BC020235 |
| Gene symbol | HSPA5 |
| Gene ID (NCBI) | 3309 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
| IHC protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
| IF protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
| IP protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
| FC protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Pharmacol Res Pharmacological preconditioning with the cellular stress inducer thapsigargin protects against experimental sepsis. | ||
Oxid Med Cell Longev Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress. | ||
J Proteomics Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration. | ||
J Virol Porcine circovirus type 2 induces ORF3-independent mitochondrial apoptosis via PERK activation and elevation of cytosolic calcium. | ||
J Neurochem Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms. | ||
Acta Biochim Biophys Sin (Shanghai) Chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and nonalcoholic steatohepatitis via angiogenesis rather than endoplasmic reticulum stress. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kevin (Verified Customer) (01-24-2019) | Works pretty good in HepG2 cells. One single band was observed.
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FH Sylvain (Verified Customer) (12-03-2018) |
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FH Natalie (Verified Customer) (11-20-2018) |
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