- Featured Product
- KD/KO Validated
GRP78/BIP Polyclonal antibody
GRP78/BIP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (8)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
369
Cat no : 11587-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, BxPC-3 cells, Caco-2 cells, HeLa cells, HepG2 cells, HT-29 cells, mouse liver tissue, rat liver tissue |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human pancreas cancer tissue, mouse kidney tissue, human breast cancer tissue, rat brain tissue, human colon cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 7 publications below |
WB | See 325 publications below |
IHC | See 51 publications below |
IF | See 46 publications below |
IP | See 8 publications below |
CoIP | See 2 publications below |
Product Information
11587-1-AP targets GRP78/BIP in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, bovine, carp, chicken, deer, fish, Hamster, medaka embryos, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GRP78/BIP fusion protein Ag2188 |
Full Name | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
Calculated molecular weight | 78 kDa |
Observed molecular weight | 70-78 kDa |
GenBank accession number | BC020235 |
Gene symbol | HSPA5 |
Gene ID (NCBI) | 3309 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
IHC protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
IF protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
IP protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Pharmacol Res Pharmacological preconditioning with the cellular stress inducer thapsigargin protects against experimental sepsis. | ||
Oxid Med Cell Longev Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress. | ||
J Proteomics Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration. | ||
J Virol Porcine circovirus type 2 induces ORF3-independent mitochondrial apoptosis via PERK activation and elevation of cytosolic calcium. | ||
J Neurochem Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms. | ||
Acta Biochim Biophys Sin (Shanghai) Chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and nonalcoholic steatohepatitis via angiogenesis rather than endoplasmic reticulum stress. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH PK (Verified Customer) (03-22-2022) |
![]() |
FH Yeawon (Verified Customer) (03-17-2022) | Works very good in mouse kidney tissue.
|
FH Emma (Verified Customer) (03-15-2022) | Works well by WB in LNCaP and CWR22Rv1 cells @ 1:1000. A single band observed.
|
FH P (Verified Customer) (05-27-2021) | Excellent.
![]() |
FH Kevin (Verified Customer) (01-24-2019) | Works pretty good in HepG2 cells. One single band was observed.
|
FH Sylvain (Verified Customer) (12-03-2018) |
|
FH Natalie (Verified Customer) (11-20-2018) |
![]() |