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  • KD/KO Validated

GRP78/BIP Polyclonal antibody

GRP78/BIP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11587-1-AP

Synonyms

BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSP A5, HSPA5, MIF2



Tested Applications

Positive WB detected inBxPC-3 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, HT-29 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue, mouse kidney tissue, rat brain tissue, mouse heart tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11587-1-AP targets GRP78/BIP in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, deer, chicken, carp, medaka embryos, monkey, fish, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP78/BIP fusion protein Ag2188
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight 78 kDa
Observed molecular weight 70-78 kDa
GenBank accession numberBC020235
Gene symbol HSPA5
Gene ID (NCBI) 3309
RRIDAB_2119855
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.

Protocols

Product Specific Protocols
WB protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IHC protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IF protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IP protocol for GRP78/BIP antibody 11587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Science

Translation from the 5' untranslated region shapes the integrated stress response.

Authors - Shelley R Starck
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
mouseWB

Crit Care

Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.

Authors - Lingbing Zhang
human,ratWB

Acta Pharm Sin B

SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.

Authors - Fen Long
human,mouseWB

J Pineal Res

Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress

Authors - Zhou Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

PK (Verified Customer) (03-22-2022)

  • Applications: Western Blot
  • Cell Tissue Type: FIBROBLAST
GRP78/BIP Antibody Western Blot validation ( dilution) in FIBROBLAST (Cat no:11587-1-AP)
FH

Yeawon (Verified Customer) (03-17-2022)

Works very good in mouse kidney tissue.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse kidney tissue
FH

Emma (Verified Customer) (03-15-2022)

Works well by WB in LNCaP and CWR22Rv1 cells @ 1:1000. A single band observed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Prostate
FH

P (Verified Customer) (05-27-2021)

Excellent.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: H9C2
GRP78/BIP Antibody Western Blot validation (1:2000 dilution) in H9C2 (Cat no:11587-1-AP)
FH

Kevin (Verified Customer) (01-24-2019)

Works pretty good in HepG2 cells. One single band was observed.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2
FH

Sylvain (Verified Customer) (12-03-2018)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: colon cancer cell lines
FH

Natalie (Verified Customer) (11-20-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Rat Dorsal Root Ganglia
GRP78/BIP Antibody Western Blot, validation (1:5000 dilution) in Rat Dorsal Root Ganglia (Cat no:11587-1-AP)