GRP78/BIP Polyclonal antibody

• Featured Product
• KD/KO Validated

GRP78/BIP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

471

Reviews (7)

Cat no : 11587-1-AP

Synonyms

BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSP A5, HSPA5, MIF2

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Validation Data Gallery

• 

  WB analysis using 11587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11587-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 11587-1-AP

  IP Result of anti-GRP78/BIP (IP:11587-1-AP, 3ug; Detection:11587-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

• 

  IHC staining of human pancreas cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat brain using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11587-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRP78/BIP antibody (11587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 11587-1-AP

  Immunofluorescent analysis of HepG2 cells using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HeLa using 11587-1-AP

  1X10^6 HeLa cells were stained with 0.2ug GRP78/BIP antibody (11587-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, HT-29 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissue, mouse kidney tissue, rat brain tissue, mouse heart tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 11 publications below
• WB: See 419 publications below
• IHC: See 62 publications below
• IF: See 59 publications below
• IP: See 8 publications below
• CoIP: See 5 publications below

Product Information

11587-1-AP targets GRP78/BIP in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine, carp, chicken, deer, fish, Hamster, medaka embryos, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRP78/BIP fusion protein Ag2188
• Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Calculated molecular weight: 78 kDa
• Observed molecular weight: 70-78 kDa
• GenBank accession number: BC020235
• Gene symbol: HSPA5
• Gene ID (NCBI): 3309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.

Publications

Species	Application	Title
	WB	Science; Translation from the 5' untranslated region shapes the integrated stress response.; Authors - Shelley R Starck
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
human,mouse	WB	J Pineal Res; Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress; Authors - Zhou Du
mouse	WB	Mol Psychiatry; Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.; Authors - M G Sharoar
mouse	WB	Sci Adv; Endoplasmic reticulum chaperone genes encode effectors of long-term memory.; Authors - Snehajyoti Chatterjee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

PK (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: FIBROBLAST

GRP78/BIP Antibody Western Blot validation ( dilution) in FIBROBLAST (Cat no:11587-1-AP)

FH

Yeawon (Verified Customer) (03-17-2022)

Works very good in mouse kidney tissue.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse kidney tissue

FH

Emma (Verified Customer) (03-15-2022)

Works well by WB in LNCaP and CWR22Rv1 cells @ 1:1000. A single band observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate

FH

P (Verified Customer) (05-27-2021)

Excellent.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: H9C2

GRP78/BIP Antibody Western Blot validation (1:2000 dilution) in H9C2 (Cat no:11587-1-AP)

FH

Kevin (Verified Customer) (01-24-2019)

Works pretty good in HepG2 cells. One single band was observed.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2

FH

Sylvain (Verified Customer) (12-03-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: colon cancer cell lines

FH

Natalie (Verified Customer) (11-20-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Rat Dorsal Root Ganglia

GRP78/BIP Antibody Western Blot, validation (1:5000 dilution) in Rat Dorsal Root Ganglia (Cat no:11587-1-AP)

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