Tested Applications
| Positive IF/ICC detected in | thapsigargin treated U2OS cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-11587 targets GRP78/BIP in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2188 Product name: Recombinant human GRP78,BIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 351-654 aa of BC020235 Sequence: LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL Predict reactive species |
| Full Name | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 70-78 kDa |
| GenBank Accession Number | BC020235 |
| Gene Symbol | GRP78/BIP |
| Gene ID (NCBI) | 3309 |
| RRID | AB_2919769 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11021 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GRP78/BIP antibody CL594-11587 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL594-11587 is a GRP78/BIP Polyclonal antibody conjugated with CoraLite® Plus 594. It has 1 total citation, published in Cell Reports (2026). The study investigated how African Swine Fever Virus (ASFV) p54 protein manipulates the secretory carrier membrane protein 3-dependent apoptotic bodies pathway to enhance cell-cell transmission, using the antibody for immunofluorescence in pig samples. The associated research fields include virology, apoptosis, and cell biology.
| Species | Application | Title |
|---|---|---|
Cell Rep ASFV p54 manipulates the secretory carrier membrane protein 3-dependent apoptotic bodies pathway to enhance cell-cell transmission. |
