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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GRP78/BIP Polyclonal antibody

GRP78/BIP Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11587

Synonyms

BIP, FLJ26106, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated GRP78/BIP Polyclonal (CL488-11587)

    IF Staining of HepG2 using CL488-11587

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 GRP78/BIP antibody (CL488-11587) at dilution of 1:100. DAPI (blue).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated GRP78/BIP Polyclonal (CL488-11587)

    FC experiment of HeLa using CL488-11587

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GRP78/BIP (CL488-11587) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11587 targets GRP78/BIP in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP78/BIP fusion protein Ag2188
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 70-78 kDa
GenBank Accession NumberBC020235
Gene Symbol HSPA5
Gene ID (NCBI) 3309
RRIDAB_2919034
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GRP78/BIP antibody CL488-11587Download protocol
FC protocol for CL Plus 488 GRP78/BIP antibody CL488-11587Download protocol
Standard Protocols
Click here to view our Standard Protocols