GRP78/BIP Monoclonal antibody
GRP78/BIP Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 66574-1-Ig
Validation Data Gallery
|Positive WB detected in
|HepG2 cells, HeLa cells, A549 cells, MCF-7 cells, Jurkat cells, HSCT6 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, SP2/0 cell, 4T1 cells
|Positive IP detected in
|Positive IHC detected in
|human liver cancer tissue, human colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66574-1-Ig targets GRP78/BIP in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, rat, mouse samples.
|Human, rat, mouse
|human, mouse, rat, zebrafish, pig
|Host / Isotype
|Mouse / IgG1
|GRP78/BIP fusion protein Ag2188
|heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.
|Product Specific Protocols
|WB protocol for GRP78/BIP antibody 66574-1-Ig
|IHC protocol for GRP78/BIP antibody 66574-1-Ig
|IF protocol for GRP78/BIP antibody 66574-1-Ig
|IP protocol for GRP78/BIP antibody 66574-1-Ig
|FC protocol for GRP78/BIP antibody 66574-1-Ig
|Click here to view our Standard Protocols
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