GRP78/BIP Monoclonal antibody
GRP78/BIP Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1D6F7
Cat no : 66574-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSCT6 cells |
Positive IP detected in | SGC-7901 cells |
Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 23 publications below |
IHC | See 1 publications below |
IF | See 8 publications below |
Product Information
66574-1-Ig targets GRP78/BIP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Reactivity | Human, rat, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | GRP78/BIP fusion protein Ag2188 |
Full Name | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
Calculated molecular weight | 78 kDa |
Observed molecular weight | 70-78 kDa |
GenBank accession number | BC020235 |
Gene symbol | HSPA5 |
Gene ID (NCBI) | 3309 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.
Protocols
Product Specific Protocols | |
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WB protocol for GRP78/BIP antibody 66574-1-Ig | Download protocol |
IHC protocol for GRP78/BIP antibody 66574-1-Ig | Download protocol |
IF protocol for GRP78/BIP antibody 66574-1-Ig | Download protocol |
IP protocol for GRP78/BIP antibody 66574-1-Ig | Download protocol |
FC protocol for GRP78/BIP antibody 66574-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Food Nutr Res Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism. | ||
Cell Death Discov ARRB1 suppresses the activation of hepatic macrophages via modulating endoplasmic reticulum stress in lipopolysaccharide-induced acute liver injury | ||
Mol Med Rep Hydroxypropyl‑β‑cyclodextrin attenuates the epithelial‑to‑mesenchymal transition via endoplasmic reticulum stress in MDA‑MB‑231 breast cancer cells. | ||
Mol Med Rep Effect of endoplasmic reticulum calcium on paraquat‑induced apoptosis of human lung type II alveolar epithelial A549 cells. | ||
Anticancer Drugs Berberine inhibits proliferation and migration of colorectal cancer cells by downregulation of GRP78. | ||
PeerJ Curcumol alleviates liver fibrosis by inducing endoplasmic reticulum stress-mediated necroptosis of hepatic stellate cells through Sirt1/NICD pathway. |