GRP78/BIP Monoclonal antibody

GRP78/BIP Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D6F7

Cat no : 66574-1-Ig

Synonyms

BIP, GRP 78, GRP78, Heat shock 70 kDa protein 5, HSPA5, MIF2



Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSCT6 cells
Positive IP detected inSGC-7901 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66574-1-Ig targets GRP78/BIP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRP78/BIP fusion protein Ag2188
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight 78 kDa
Observed molecular weight 70-78 kDa
GenBank accession numberBC020235
Gene symbol HSPA5
Gene ID (NCBI) 3309
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

Protocols

Product Specific Protocols
WB protocol for GRP78/BIP antibody 66574-1-IgDownload protocol
IHC protocol for GRP78/BIP antibody 66574-1-IgDownload protocol
IF protocol for GRP78/BIP antibody 66574-1-IgDownload protocol
IP protocol for GRP78/BIP antibody 66574-1-IgDownload protocol
FC protocol for GRP78/BIP antibody 66574-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Food Nutr Res

Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism.

Authors - Meilan Xue
humanWB

Mol Med Rep

Hydroxypropyl‑β‑cyclodextrin attenuates the epithelial‑to‑mesenchymal transition via endoplasmic reticulum stress in MDA‑MB‑231 breast cancer cells.

Authors - Yifan Wu
humanWB,IF

Anticancer Drugs

Berberine inhibits proliferation and migration of colorectal cancer cells by downregulation of GRP78.

Authors - Chenxue Gong
humanWB

Int J Mol Med

PMID: 31432100

Authors - Zhiqiang He
humanWB,IHC,IF

Biochem Biophys Res Commun

TFIIB-related factor 2 regulates glucose-regulated protein 78 expression in acquired middle ear cholesteatoma.

Authors - RongCui Sui
humanWB

Brain Res Bull

PMID: 32544512

Authors - Yi Liu