GRP78/BIP Monoclonal antibody

GRP78/BIP Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, FC

Conjugate

Unconjugated

Publications

16

CloneNo.

1D6F7

Cat no : 66574-1-Ig

Synonyms

BIP, GRP 78, GRP78, Heat shock 70 kDa protein 5, HSPA5, MIF2

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Validation Data Gallery

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  WB analysis using 66574-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of SGC-7901 using 66574-1-Ig

  IP result of anti-GRP78/BIP (IP:66574-1-Ig, 5ug; Detection:66574-1-Ig 1:3000) with SGC-7901 cells lysate 2200 ug.

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  IHC staining of human liver cancer using 66574-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66574-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66574-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of MCF-7 using 66574-1-Ig

  1X10^6 MCF-7 cells were stained with 0.2 ug Anti-Human GRP78/BIP (66574-1-Ig, Clone:1D6F7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSCT6 cells
• Positive IP detected in: SGC-7901 cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: MCF-7 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Product Information

66574-1-Ig targets GRP78/BIP in WB, IP, IHC, IF, FC applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRP78/BIP fusion protein Ag2188
• Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Calculated molecular weight: 78 kDa
• Observed molecular weight: 70-78 kDa
• GenBank accession number: BC020235
• Gene symbol: HSPA5
• Gene ID (NCBI): 3309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

Publications

Species	Application	Title
mouse	WB	Food Nutr Res; Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism.; Authors - Meilan Xue
mouse	IF	Cell Death Discov; ARRB1 suppresses the activation of hepatic macrophages via modulating endoplasmic reticulum stress in lipopolysaccharide-induced acute liver injury; Authors - Yiming Lei
human	WB	Mol Med Rep; Hydroxypropyl‑β‑cyclodextrin attenuates the epithelial‑to‑mesenchymal transition via endoplasmic reticulum stress in MDA‑MB‑231 breast cancer cells.; Authors - Yifan Wu
human	WB,IF	Anticancer Drugs; Berberine inhibits proliferation and migration of colorectal cancer cells by downregulation of GRP78.; Authors - Chenxue Gong
human	WB	Int J Mol Med; PMID: 31432100; Authors - Zhiqiang He
human	WB,IHC,IF	Biochem Biophys Res Commun; TFIIB-related factor 2 regulates glucose-regulated protein 78 expression in acquired middle ear cholesteatoma.; Authors - RongCui Sui