|Positive WB detected in||A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSCT6 cells|
|Positive IP detected in||SGC-7901 cells|
|Positive IHC detected in||human liver cancer tissue, human colon cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66574-1-Ig targets GRP78/BIP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.
|Tested Reactivity||Human, rat, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GRP78/BIP fusion protein Ag2188|
|Full Name||heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||70-78 kDa|
|GenBank accession number||BC020235|
|Gene ID (NCBI)||3309|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.
|Product Specific Protocols|
|WB protocol for GRP78/BIP antibody 66574-1-Ig||Download protocol|
|IHC protocol for GRP78/BIP antibody 66574-1-Ig||Download protocol|
|IF protocol for GRP78/BIP antibody 66574-1-Ig||Download protocol|
|IP protocol for GRP78/BIP antibody 66574-1-Ig||Download protocol|
|FC protocol for GRP78/BIP antibody 66574-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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