Filter: AllWBIPIHCIF/ICC

WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)
Various cells were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
IP experiment of SGC-7901 using 66574-1-Ig (same clone as 66574-1-PBS)
IP result of anti-GRP78/BIP (IP:66574-1-Ig, 5ug; Detection:66574-1-Ig 1:3000) with SGC-7901 cells lysate 2200 ug. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66574-1-Ig (same clone as 66574-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66574-1-Ig (same clone as 66574-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66574-1-Ig (same clone as 66574-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66574-1-Ig (same clone as 66574-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 66574-1-Ig (same clone as 66574-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed thapsigargin treated U2OS cells using GRP78/BIP antibody (66574-1-Ig, Clone: 1D6F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

GRP78/BIP Monoclonal antibody, PBS Only

GRP78/BIP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 66574-1-PBS

Clone No.1D6F7

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

BIP, GRP78, HSPA5, 1D6F7, 78 kDa glucose-regulated protein

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66574-1-PBS targets GRP78/BIP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag2188 Product name: Recombinant human GRP78,BIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 351-654 aa of BC020235 Sequence: LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL Predict reactive species
Full Name	heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated Molecular Weight	78 kDa
Observed Molecular Weight	70-78 kDa
GenBank Accession Number	BC020235
Gene Symbol	GRP78/BIP
Gene ID (NCBI)	3309
RRID	AB_2881934
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P11021
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.