CoraLite®488-conjugated GRP78/BIP Monoclonal antibody

GRP78/BIP Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1D6F7

Cat no : CL488-66574

Synonyms

BIP, FLJ26106, GRP 78, GRP78, Heat shock 70 kDa protein 5, HSPA5, MIF2



Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells
For other applications, we recommend the unconjugated version of this antibody, 66574-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

CL488-66574 targets GRP78/BIP in WB applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRP78/BIP fusion protein Ag2188
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight 78 kDa
Observed molecular weight78 kDa
GenBank accession numberBC020235
Gene symbol HSPA5
Gene ID (NCBI) 3309
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

Protocols

Product Specific Protocols
WB protocol for CL488 GRP78/BIP antibody CL488-66574Download protocol
Standard Protocols
Click here to view our Standard Protocols