CoraLite® Plus 647-conjugated GRP78/BIP Monoclonal antibody

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  FC experiment of HeLa using CL647-66574

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated GRP78/BIP Monoclonal antibody (CL647-66574, Clone:1D6F7)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

GRP78/BIP Monoclonal Antibody for FC (Intra)

Cat No. CL647-66574

Clone No.1D6F7

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

FC (Intra)

Synonyms

BIP, FLJ26106, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66574 targets GRP78/BIP in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2188

  Product name: Recombinant human GRP78,BIP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 351-654 aa of BC020235

  Sequence: LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL

• Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC020235
• Gene Symbol: GRP78/BIP
• Gene ID (NCBI): 3309
• RRID: AB_2935062
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P11021
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.