GSG2 Polyclonal antibody

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  WB analysis using 55454-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55454-1-AP (GSG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GSG2 Polyclonal Antibody for WB, ELISA

Cat No. 55454-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

H haspin, HASPIN

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Tested Applications

• Positive WB detected in: HEK-293T cells, K-562 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55454-1-AP targets GSG2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: germ cell associated 2 (haspin)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: NM_031965
• Gene Symbol: GSG2
• Gene ID (NCBI): 83903
• RRID: AB_3086451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TF76
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Haploid germ cell-specific nuclear protein kinase (Haspin, alias GSG2) is an atypical eukaryotic protein kinase that was for a long time considered an inactive pseudokinase due to the low degree of structural homology of GSG2 with the 'classical' protein kinases (PMID: 29698892). GSG2 is a serine/threonine kinase that associates with chromosomes and phosphorylates threonine 3 of histone 3 during mitosis (PMID: 32439830).