GSK3A Polyclonal antibody

GSK3A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13419-1-AP

Synonyms

GSK3 alpha, GSK3A



Tested Applications

Positive WB detected inhuman serum tissue, mouse liver tissue
Positive IHC detected inhuman prostate cancer tissue, human lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

13419-1-AP targets GSK3A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSK3A fusion protein Ag4168
Full Name glycogen synthase kinase 3 alpha
Calculated molecular weight 483 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC027984
Gene symbol GSK3A
Gene ID (NCBI) 2931
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase kinase-3 alpha (GSKA) is one of the two functional isoforms encoded by the GSK gene and 

acts as a tumor suppressor through its kinase function, targeting many different proteins.


What is the molecular weight of GSKA?

51 kDa. GSKA is a protein serine kinase composed of 483 amino acids and the catalytic domain shares a high 

degree of homology with GSKB, although they are encoded on separate genes. 


What is the function of GSKA?

Constitutively expressed across all tissues, GSKA has a large number of target proteins and is therefore involved 

in many different pathways (PMID: 19923896). It was first identified as a regulator of glycogen metabolism, as 

GSK3A phosphorylates the enzyme glycogen synthase, thereby inhibiting glycogenesis, the conversion of glucose 

into glycogen (PMID: 6249596). It has since been shown to interact with many other proteins and is one of the 

kinases with most substrates in the cell, with roles in cell cycle progression, proliferation, differentiation, and 

apoptosis (PMID: 17570479; PMID: 11563964). Typically, GSKA negatively regulates its downstream substrates, 

often tagging proteins for ubiquitination. It has important roles in the Wnt and PI3K signaling pathways.


What is the role of GSK3A in neurodegenerative disease?

GSK3A is known to interact with beta-amyloid and tau, proteins that aggregate in areas of the neuronal system 

as part of the pathology of neurodegenerative diseases such as Alzheimer’s disease. It has been shown that 

GSK3A causes abnormal phosphorylation of the microtubule-binding protein tau in a way that reflects the 

pathology of Alzheimer’s disease (PMID: 1334849; PMID: 1336152). GSKA has also been shown to process 

amyloid precursor protein to beta-amyloid, another protein that forms the inclusion bodies, or Lewy bodies, 

that are one of the hallmarks in Alzheimer’s (PMID: 1276154). Another protein involved in the pathology of 

Alzheimer’s is α-synuclein, which is known to be a substrate for GSK-3β phosphorylation (PMID: 24681994).


Protocols

Product Specific Protocols
WB protocol for GSK3A antibody 13419-1-APDownload protocol
IHC protocol for GSK3A antibody 13419-1-APDownload protocol
IF protocol for GSK3A antibody 13419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Pathol Oncol Res

Knockdown of Sox2 Inhibits OS Cells Invasion and Migration via Modulating Wnt/β-Catenin Signaling Pathway.

Authors - Liang Tang
humanWB

J Cell Physiol

The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.

Authors - Nan Song
mouseWB

Neuropsychopharmacology

Upregulation of GSK3β Contributes to Brain Disorders in Elderly REGγ-knockout Mice.

Authors - Yiqing Lv