eRF3a/GSPT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

eRF3a/GSPT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 10763-1-AP

Synonyms

GSPT1, eRF3a, EC:3.6.5.-, G1 to S phase transition 1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A

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Validation Data Gallery

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  WB analysis using 10763-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10763-1-AP

  WB result of eRF3a/GSPT1 antibody (10763-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-eRF3a/GSPT1 transfected HepG2 cells.

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  WB analysis using 10763-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10763-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10763-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10763-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using eRF3a/GSPT1 antibody (10763-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, Caco-2 cells, HCT 116 cells, HeLa cells, MOLT-4 cells
• Positive IHC detected in: human liver cancer tissue, mouse brain tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

10763-1-AP targets eRF3a/GSPT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: eRF3a/GSPT1 fusion protein Ag1184
• Full Name: G1 to S phase transition 1
• Calculated Molecular Weight: 68 aa, 4 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC009503
• Gene Symbol: GSPT1
• Gene ID (NCBI): 2935
• RRID: AB_2115506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. eRF3a, also known as GSPT1, is one subunit of eRF3(PMID:15917414,12923185). It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1. eRF3a/GSPT1 exists some isoforms with MV 69 kDa and 56 kDa. Identification of a processed form of eRF3a/GSPT1 as a BIR3-binding protein-Using a GST-BIR3 fusion protein as an affinity reagent to purify new IAP binding proteins from extracts of human cells and mouse tissues, we previously isolated 3 proteins of molecular weights 23, 38 and 80 kDa. 80 kDa band confirmed that it is a processed form of the human GSPT1/eRF3a protein, lacking the first 69 residues(PMID: 12865429).

Publications

Species	Application	Title
human	WB	Nature; A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase.; Authors - Mary E Matyskiela ; KD Validated
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human, mouse	WB	Mol Cell; Mammalian hyperplastic discs homolog EDD regulates miRNA-mediated gene silencing.; Authors - Hong Su
human	WB	Cell Rep Med; Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition; Authors - Yu Chang
human	WB,IF	Cardiovasc Res; Readthrough of nonsense mutation W822X in the SCN5A gene can effectively restore expression of cardiac Na+ channels.; Authors - Teng Siyong S ; KD Validated
human	WB	J Biol Chem; A Novel Rac1-GSPT1 Signaling Pathway Controls Astrogliosis Following Central Nervous System Injury.; Authors - Taiji Ishii