Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, MOLT-4 cells, Caco-2 cells, HCT 116 cells, HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human liver cancer tissue, mouse brain tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10763-1-AP targets eRF3a/GSPT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1184 Product name: Recombinant human GSPT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 265-633 aa of BC009503 Sequence: QEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD Predict reactive species |
| Full Name | G1 to S phase transition 1 |
| Calculated Molecular Weight | 68 aa, 4 kDa |
| Observed Molecular Weight | 80-85 kDa |
| GenBank Accession Number | BC009503 |
| Gene Symbol | GSPT1 |
| Gene ID (NCBI) | 2935 |
| RRID | AB_2115506 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15170 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. eRF3a, also known as GSPT1, is one subunit of eRF3(PMID:15917414,12923185). It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1. eRF3a/GSPT1 exists some isoforms with MV 69 kDa and 56 kDa. Identification of a processed form of eRF3a/GSPT1 as a BIR3-binding protein-Using a GST-BIR3 fusion protein as an affinity reagent to purify new IAP binding proteins from extracts of human cells and mouse tissues, we previously isolated 3 proteins of molecular weights 23, 38 and 80 kDa. 80 kDa band confirmed that it is a processed form of the human GSPT1/eRF3a protein, lacking the first 69 residues(PMID: 12865429).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for eRF3a/GSPT1 antibody 10763-1-AP | Download protocol |
| IHC protocol for eRF3a/GSPT1 antibody 10763-1-AP | Download protocol |
| WB protocol for eRF3a/GSPT1 antibody 10763-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody 10763-1-AP (anti-GSPT1/eRF1, affinity purification) has 30 citations published in journals including Nature, Cancer Cell, Molecular Cell, Journal of Medicinal Chemistry, Cell Reports Medicine, and Current Medical Sciences. Associated research fields span PROTAC/molecular glue degrader development, hematological and solid tumor oncology, translation termination mechanisms, neurodegenerative disease, cardiac electrophysiology, stress granule biology, and miRNA/lncRNA-mediated gene regulation.
| Species | Application | Title |
|---|---|---|
Nature A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase.
| ||
J Hematol Oncol Novel potent molecular glue degraders against broad range of hematological cancer cell lines via multiple neosubstrates degradation | ||
Mol Cancer Nicotine-mediated invasion and migration of non-small cell lung carcinoma cells by modulating STMN3 and GSPT1 genes in an ID1-dependent manner. | ||
Cancer Res Single-Cell Lineage Tracing Uncovers Resistance Signatures and Sensitizing Strategies to FLT3 Inhibitors in Acute Myeloid Leukemia.
| ||
Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. |
