Tested Applications
| Positive WB detected in | HepG2 cells, HUVEC cells, Raji cells, L02 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human testis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14535-1-AP targets GSTK1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6036 Product name: Recombinant human GSTK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC050715 Sequence: MGPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARL Predict reactive species |
| Full Name | glutathione S-transferase kappa 1 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC050715 |
| Gene Symbol | GSTK1 |
| Gene ID (NCBI) | 373156 |
| RRID | AB_2115910 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2Q3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity in mice and human, hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GSTK1 antibody 14535-1-AP | Download protocol |
| IP protocol for GSTK1 antibody 14535-1-AP | Download protocol |
| WB protocol for GSTK1 antibody 14535-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSTK1 Polyclonal antibody (Cat# 14535-1-AP) has 22 citations spanning journals including Autophagy, J Exp Clin Cancer Res, Cardiovasc Res, Sci Total Environ, Apoptosis, Mol Med, Free Radic Biol Med, Biochem Pharmacol, Life Sci, Int Immunopharmacol, iScience, FASEB J, J Clin Med, and PLoS One. Research fields cover diabetic nephropathy, hepatocellular carcinoma, cardiac injury, ferroptosis, colon cancer, gestational diabetes, pulmonary fibrosis, preeclampsia, sepsis, aging, glioma, leukemia/lymphoma, and kidney stone formation.
| Species | Application | Title |
|---|---|---|
Autophagy GSTK1 and RETREG1/FAM134B-mediated reticulophagy attenuates tubular injury in diabetic nephropathy through endoplasmic reticulum stress and apoptosis | ||
J Exp Clin Cancer Res GSTK1 suppresses HCC aggravation via L-carnitine metabolism by PGAM5/DRP1 complex-mediated mitochondrial quality control. | ||
Cardiovasc Res Depleting inositol pyrophosphate 5-InsP7 protected the heart against ischaemia-reperfusion injury by elevating plasma adiponectin | ||
Sci Total Environ m6A methylation-mediated PGC-1α contributes to ferroptosis via regulating GSTK1 in arsenic-induced hepatic insulin resistance | ||
Apoptosis KAT2A alleviates the glucose starvation-induced mitochondrial oxidative stress and ferroptosis to promote colon cancer progression. | ||
Mol Med Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet |
Reviews
What customers say
All three reviews are for Western Blot applications, with ratings of 5, 4, and 5 out of 5. Users consistently report strong, specific signal and describe the product as "excellent" and "very good." One reviewer used a 1:1000 dilution on A549 cells. Overall, customers express high satisfaction with the antibody's performance and specificity in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
S (Verified Customer) (08-15-2024) | Excellent
 |
SD (Verified Customer) (07-05-2024) | Very good
 |
Andrea (Verified Customer) (01-23-2023) | Strong and specific signal.
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