GSTO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GSTO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 15124-1-AP

Synonyms

Glutathione S-transferase omega-1, Glutathione S-transferase omega 1-1, glutathione S transferase omega 1, EC:2.5.1.18, EC:1.8.5.1

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Validation Data Gallery

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  WB analysis using 15124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15124-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15124-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15124-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 15124-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15124-1-AP

  IP result of anti-GSTO1 (IP:15124-1-AP, 4ug; Detection:15124-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human pancreas cancer using 15124-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15124-1-AP (GSTO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15124-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15124-1-AP (GSTO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 15124-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GSTO1 antibody (15124-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, rat brain tissue, mouse liver tissue, human heart tissue, U-937 cells, Jurkat cells, PC-3 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

15124-1-AP targets GSTO1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTO1 fusion protein Ag7199
• Full Name: glutathione S-transferase omega 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC000127
• Gene Symbol: GSTO1
• Gene ID (NCBI): 9446
• RRID: AB_2248132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells. Human GSTO1 assembles as a dimer. This antibody detects both monomer (27-30 kDa) and dimer forms of GSTO1 (54-64 kDa). (PMID: 21495794)

Publications

Species	Application	Title
human	WB	Mol Cell; SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation; Authors - Wenxin Zhang
mouse	WB,IF	J Exp Med; ASC deglutathionylation is a checkpoint for NLRP3 inflammasome activation.; Authors - Shuhang Li ; KO Validated
pig	WB	Food Chem; TMT-based quantitative proteomic analysis of porcine muscle associated with postmortem meat quality.; Authors - Xinhua Hou
		Biochem J; Selenophosphate Synthetase 1 is an Essential Protein with Roles in Regulation of Redox Homeostasis in Mammals.; Authors - Ryuta Tobe
mouse	WB	Toxicol Appl Pharmacol; PMID: 24998975; Authors - Jing-Jing Chuang
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang