Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse liver tissue, human heart tissue, U-937 cells, Jurkat cells, PC-3 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15124-1-AP targets GSTO1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GSTO1 fusion protein Ag7199|
|Full Name||glutathione S-transferase omega 1|
|Calculated molecular weight||28 kDa|
|Observed molecular weight||27-30 kDa|
|GenBank accession number||BC000127|
|Gene ID (NCBI)||9446|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells. Human GSTO1 assembles as a dimer. This antibody detects both monomer (27-30 kDa) and dimer forms of GSTO1 (54-64 kDa). (PMID: 21495794)
|Product Specific Protocols|
|WB protocol for GSTO1 antibody 15124-1-AP||Download protocol|
|IHC protocol for GSTO1 antibody 15124-1-AP||Download protocol|
|IF protocol for GSTO1 antibody 15124-1-AP||Download protocol|
|IP protocol for GSTO1 antibody 15124-1-AP||Download protocol|
|Click here to view our Standard Protocols|
GSTO1 acts as a mediator in sodium fluoride-induced alterations of learning and memory related factors expressions in the hippocampus cell line.
Biochem Biophys Res Commun
Inhibition of NRF2 signaling and increased reactive oxygen species during embryogenesis in a rat model of retinoic acid-induced neural tube defects.
The interaction of arsenic and N-butyl-N-(4-hydroxybutyl)nitrosamine on urothelial carcinogenesis in mice.
Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.
Toxicol Appl Pharmacol