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  • KD/KO Validated

GSTO1 Polyclonal antibody

GSTO1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15124-1-AP

Synonyms

GSTO 1, GSTO1, GSTTLp28, P28



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, human heart tissue, U-937 cells, Jurkat cells, PC-3 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15124-1-AP targets GSTO1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTO1 fusion protein Ag7199
Full Name glutathione S-transferase omega 1
Calculated molecular weight 28 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC000127
Gene symbol GSTO1
Gene ID (NCBI) 9446
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells. Human GSTO1 assembles as a dimer. This antibody detects both monomer (27-30 kDa) and dimer forms of GSTO1 (54-64 kDa). (PMID: 21495794)

Protocols

Product Specific Protocols
WB protocol for GSTO1 antibody 15124-1-APDownload protocol
IHC protocol for GSTO1 antibody 15124-1-APDownload protocol
IF protocol for GSTO1 antibody 15124-1-APDownload protocol
IP protocol for GSTO1 antibody 15124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Chemosphere

GSTO1 acts as a mediator in sodium fluoride-induced alterations of learning and memory related factors expressions in the hippocampus cell line.

Authors - Jinming Wang
mouseWB,IF

Biochem Biophys Res Commun

PMID: 32057363

Authors - Linlin Wang
  • KO Validated
ratWB

Neurotoxicology

Inhibition of NRF2 signaling and increased reactive oxygen species during embryogenesis in a rat model of retinoic acid-induced neural tube defects.

Authors - Dan Liu
mouseWB

PLoS One

The interaction of arsenic and N-butyl-N-(4-hydroxybutyl)nitrosamine on urothelial carcinogenesis in mice.

Authors - Yuan-Chang Dai
humanWB

J Proteomics

Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.

Authors - Dan Zhang
mouseWB

Toxicol Appl Pharmacol

PMID: 24998975

Authors - Jing-Jing Chuang